重组Anti-ATF1抗体[EPR1590(2)] (ab134104)
![Western blot - Anti-ATF1 antibody [EPR1590(2)] (ab134104)](https://www.abcam.cn/ps/products/134/ab134104/Images/ab134104-148281-anti-atf1-antibody-epr15902-western-blot.jpg "Western blot - Anti-ATF1 antibody [EPR1590(2)] (ab134104)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1590(2)] to ATF1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ATF1抗体[EPR1590(2)]
参阅全部 ATF1 一抗 -
描述
兔单克隆抗体[EPR1590(2)] to ATF1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- K562, HeLa, and HepG2 whole cell lysate (ab7900)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1590(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134104于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
|
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| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa). |
|
IP
1/10 - 1/100. |
应用说明
Is unsuitable for Flow Cyt or IHC-P.
靶标
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功能
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. -
疾病相关
Defects in ATF1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein. -
序列相似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 466 Human
- Omim: 123803 Human
- SwissProt: P18846 Human
- Unigene: 648565 Human
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别名
- Activating transcription factor 1 antibody
- ATF 1 antibody
- atf1 antibody
see all
图片
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Western blot - Anti-ATF1 antibody [EPR1590(2)] (ab134104)All lanes : Anti-ATF1 antibody [EPR1590(2)] (ab134104) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
![Anti-ATF1 antibody [EPR1590(2)] (ab134104)](https://www.abcam.cn/ps/products/134/ab134104/Images/ab134104-2-benefits-of-recombinant-antibodies.png)
Anti-ATF1 antibody [EPR1590(2)] (ab134104)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab134104 被引用在 5 文献中.
- Möller E et al. EWSR1-ATF1 dependent 3D connectivity regulates oncogenic and differentiation programs in Clear Cell Sarcoma. Nat Commun 13:2267 (2022). PubMed: 35477713
- Ascenção K et al. Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells. Redox Biol 56:102466 (2022). PubMed: 36113340
- Yang SC et al. Down-regulation of ATF1 leads to early neuroectoderm differentiation of human embryonic stem cells by increasing the expression level of SOX2. FASEB J 33:10577-10592 (2019). PubMed: 31242772
- Vilaboa N et al. New inhibitor targeting human transcription factor HSF1: effects on the heat shock response and tumor cell survival. Nucleic Acids Res 45:5797-5817 (2017). WB ; Human . PubMed: 28369544
- Yamada K et al. EWS/ATF1 expression induces sarcomas from neural crest-derived cells in mice. J Clin Invest 123:600-10 (2013). WB ; Mouse . PubMed: 23281395