Anti-ATF-4抗体- C-terminal (ab1371)

Reviewing purchases of this product, the lab purchased ab1371 and not ab50546. Furthermore, in the methods section of the paper an anti-goat secondary antibody is mentioned but no goat primary is stated as being used. Therefore we do believe the researchers did use ab1371 but incorrectly cited this in the paper.

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Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande du remplacement gratuit de ce produit : xxxxxx. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

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Thank you for getting back to me. I am sorry that I did not provide you with reference to the positive controls for each of these antibodies. The following positive control should be used for ATF4 antibody (ab1371): Raji cell lysate: ab7908 Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate The following positive controls are recommended for use with p21 antibody (ab7960): C32 nuclear lysate or Colon carcinoma. Unfortunately we do not sell either of these as lysates. I am sorry I cannot be of more assistance on this part. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your phone call and I apologize for the shortage that you received. I have arranged for a free of charge replacement vial to be sent to you. It is being shipped to your attention on order reference # 99647 and you should receive this tomorrow. Please let me know if you have any additional questions or concerns.

I'm sorry to hear you've had trouble with ab1371. Thank you for taking the time to fill in the self-help section to contact us, the information you have given me is very useful. I think you have taken all the right steps to get the antibody working and I will arrange a refund for this antibody immediately. As you mentioned, a number of customers have reported similar results in their preparations and we will investigate this further. Once again my sincere apologies,

Thank you for your enquiry and providing more details of your assay. As we understand from your e-mail, the main problem you are experiencing is that you have a lot of non-specific bands. We have done our Western blot analysis with rabbit anti-goat IgG HRP and have only one distinct band at around 40 kDa (using Raji cell lysate). As we have indicated in our previous e-mail, we have not tested this primary antibody in hamster so we do not now if it works in this species. Reading through your protocol we see that you use 5% non-fat dry milk for blocking and a bovine anti-goat IgG conjugated with HRP as secondary antibody. Since the blocking agent is milk and the secondary antibody was raised in bovine, it is probable that there is a cross-reaction. The other alternative is that cow (bovine) and goat - these two species - phylogenetically are not very different from each other and the secondary antibody cross-reacting with endogenous immunoglobulins in the sample For more information about how to choose an antibody, please take a look at the following website: https://www.abcam.com/index.html?pageconfig=tech&mode=choosing Therefore we would suggest testing the secondary antibody alone (run a “secondary control” without the primary antibody) to see if you still have non-specific bands. We would also recommend using either a rabbit or a donkey polyclonal secondary antibody instead of bovine one. For your peace of mind, in case there may be some problem with the primary antibody, certainly we can offer you a replacement vial. If you would like to get it, then please do let us know and confirm your Purchase Order Number or Abcam Order Number. We are looking forward to hearing from you soon.

Thank you for your message. As we understand from your e-mail you say that "hamster proteins are usually very similar to rat and mouse proteins". However, we would like to emphasize the fact that we haven't tested this particular antibody on hamster. It is possible that there is some cross-reaction with a 100 kDa protein in hamster that doesn't happen in human. All tested applications are specified on Abcam product datasheets. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.