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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper

Anti-ATF-4抗体- C-terminal (ab1371)

  • Datasheet
Reviews (3)Q&A (10)References (19)

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Western blot - Anti-ATF-4 antibody - C-terminal (ab1371)

    Key features and details

    • Goat polyclonal to ATF-4 - C-terminal
    • Suitable for: WB
    • Reacts with: Mouse, Human, Pig
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-ATF-4抗体- C-terminal
      参阅全部 ATF-4 一抗
    • 描述

      山羊多克隆抗体to ATF-4 - C-terminal
    • 宿主

      Goat
    • 特异性

      This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Human, Pig
      预测可用于: Rat, Cow
    • 免疫原

      Synthetic peptide corresponding to Human ATF-4 aa 339-351 (C terminal) (Cysteine residue).
      Sequence:

      C-EEVRKARGKKRVP


      Database link: P18848
      (Peptide available as ab22854)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • WB: Mouse heart lysate
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.05% Tris, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • Leucine Zipper

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • K-562 whole cell lysate (ab7911)
    • Recombinant Protein

      • Recombinant Human ATF-4 protein (ab109946)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1371于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (2)
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 37 kDa).

    1 hour primary incubation is recommended for this product.

    说明
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 37 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
    • 序列相似性

      Belongs to the bZIP family.
      Contains 1 bZIP domain.
    • 细胞定位

      Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.
    • Target information above from: UniProt accession P18848 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 509107 Cow
      • Entrez Gene: 468 Human
      • Entrez Gene: 11911 Mouse
      • Entrez Gene: 79255 Rat
      • Omim: 604064 Human
      • SwissProt: Q3ZCH6 Cow
      • SwissProt: P18848 Human
      • SwissProt: Q06507 Mouse
      • SwissProt: Q9ES19 Rat
      • Unigene: 496487 Human
      • Unigene: 641 Mouse
      • Unigene: 2423 Rat
      see all
    • 别名

      • Activating transcription factor 4 antibody
      • ATF 4 antibody
      • ATF4 antibody
      • ATF4 protein antibody
      • ATF4_HUMAN antibody
      • cAMP-dependent transcription factor ATF-4 antibody
      • cAMP-responsive element-binding protein 2 antibody
      • CREB 2 antibody
      • CREB-2 antibody
      • CREB2 antibody
      • Cyclic AMP dependent transcription factor ATF 4 antibody
      • Cyclic AMP response element binding protein 2 antibody
      • Cyclic AMP-dependent transcription factor ATF-4 antibody
      • Cyclic AMP-responsive element-binding protein 2 antibody
      • DNA binding protein TAXREB67 antibody
      • DNA-binding protein TAXREB67 antibody
      • Tax Responsive Enhancer Element B67 antibody
      • Tax-responsive enhancer element-binding protein 67 antibody
      • TaxREB67 antibody
      • TXREB antibody
      see all

    图片

    • Western blot - Anti-ATF-4 antibody - C-terminal (ab1371)
      Western blot - Anti-ATF-4 antibody - C-terminal (ab1371)
      Anti-ATF-4 antibody - C-terminal (ab1371) at 1 µg/ml + Mouse Heart lysate at 35 µg

      Predicted band size: 37 kDa
      Observed band size: 35-37 kDa why is the actual band size different from the predicted?



      Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (19)

    发表研究结果有使用 ab1371?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1371 被引用在 19 文献中.

    • Xu R  et al. The breast cancer oncogene IKKe coordinates mitochondrial function and serine metabolism. EMBO Rep 21:e48260 (2020). PubMed: 32783398
    • Roberson PA  et al. LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle. Front Nutr 7:628405 (2020). PubMed: 33521042
    • Yi H  et al. Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury via Modulating PKC-ß/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways. Antioxid Redox Signal 30:1949-1968 (2019). PubMed: 30484323
    • Zhao YS  et al. Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats. Oxid Med Cell Longev 2019:7415212 (2019). PubMed: 30984338
    • Huang T  et al. Curcumin mitigates axonal injury and neuronal cell apoptosis through the PERK/Nrf2 signaling pathway following diffuse axonal injury. Neuroreport 29:661-677 (2018). PubMed: 29570500
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 13 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ATF-4 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (vascular smooth muscle cells)
    Permeabilization
    Yes - 0.1 % triton X-100
    Specification
    vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Methanol:Acetone 1:1
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 29 2015

    Western blot abreview for Anti-ATF4 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (kidney)
    Loading amount
    30 µg
    Specification
    kidney
    Gel Running Conditions
    Reduced Denaturing (4-12 %)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 15 2012

    Western blot abreview for Anti-ATF4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3)
    Loading amount
    50 µg
    Specification
    3T3
    Treatment
    Cells transfected with plasmid encoding ATF4 with HA tag
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 12 2011

    Question

    Should the reference (PMID19747230) be for ab50546 and not ab1371?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 31 2013

    Answer

    Reviewing purchases of this product, the lab purchased ab1371 and not ab50546. Furthermore, in the methods section of the paper an anti-goat secondary antibody is mentioned but no goat primary is stated as being used. Therefore we do believe the researchers did use ab1371 but incorrectly cited this in the paper.

    Read More

    Karen Baker

    Abcam Scientific Support

    回复于 Jul 31 2013

    Question

    Plainte pour ab1371 en western blot.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 18 2012

    Answer

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande du remplacement gratuit de ce produit : xxxxxx. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    N'hésitez pas à nous contacter lors d'une prochaine occasion.

    Read More

    Abcam Scientific Support

    回复于 Dec 18 2012

    Question

    Hello,thank you for your reply. There was a second question that you did not touch on.I need to buy positive controls for both antibodies namelly C32 nuclear lysate and Raji cell lysate. Could you please e mail me cataloug numbers for them and if possible soon because I would like to place an order today. Thank you In response to your enquiry (ref: 760868):I have trouble using P 21 and ATF-4 antibodies(from other supplieres) in a western blot of mouse heart protein extracts. All literature mentiones cell extracts. I would like to order these 2 antibodies from your company. What is your experience using them on tissue extracts in Western blots?Where can I get the appropriate positive controls listed on data sheets? We have an answer Thank you for your enquiry.I am pleased that you are considering purchasing our p21 and ATF-4 antibodies. With regards the application of these antibodies using tissue lysates; p21 antibody (ab7960) have been applied by Xiaobo Chen using human smooth muscle cells. He has left a favourable review as have many of our customers who have applied this antibody using western blot against whole cell lysates. It has been shown to recognize murine p21 in a broad range of applications.ATF4 antibody (ab1371) has been shown to work by western blotting. It is predicted to cross react with the murine homolog based on sequence homology.I hope this information helps, please do not hesitate to contact us if you need any more advice or information.You can return to the datasheet for the product by clicking on:https://www.abcam.com/go.cfmp=4&ab=7960&v=52365

    Read More

    Abcam community

    Verified customer

    Asked on Jan 27 2006

    Answer

    Thank you for getting back to me. I am sorry that I did not provide you with reference to the positive controls for each of these antibodies. The following positive control should be used for ATF4 antibody (ab1371): Raji cell lysate: ab7908 Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate The following positive controls are recommended for use with p21 antibody (ab7960): C32 nuclear lysate or Colon carcinoma. Unfortunately we do not sell either of these as lysates. I am sorry I cannot be of more assistance on this part. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jan 27 2006

    Question

    Customer reported receiving 174 ul instead of 200 ul in his vial of ab1371. Lot# 117693; Order# 98728.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 31 2005

    Answer

    Thank you for your phone call and I apologize for the shortage that you received. I have arranged for a free of charge replacement vial to be sent to you. It is being shipped to your attention on order reference # 99647 and you should receive this tomorrow. Please let me know if you have any additional questions or concerns.

    Read More

    Abcam Scientific Support

    回复于 Aug 31 2005

    Question

    BATCH NUMBER 63832 ORDER NUMBER 49259 DESCRIPTION OF THE PROBLEM Non-specific band. In addition to a high background (we are using the Abcam anti-goat secondary antibody raised in donkey) we do not see any band of the expeted size. We have several bands as it seems to see the case for other users. The most intense of them are approx. at 100 Kda, 60 Kda and 55 Kda...but anything at all between 40-45. SAMPLE We are using whole cell extracts from rat C6 glioma PRIMARY ANTIBODY We tried two different dilutions of primary antibody 1:500 and 1:200 in TBS-t 0.1%. We also peformed three different conditions for incubation with 1ry antibody in three different membranes: 1 h room temperature, 4?C o/n and 4?C o/n plus 1 h room temperature. Washes after primary antibody incubation: 2 quick washes, 1x10' and 2x5' with TBSt 0.1 % SECONDARY ANTIBODY We used the abcam anti-goat 2ry atibody raised in donkey (1:2000) in TBSt DETECTION METHOD Home made ECL (we regularly eploy it in WB) SAMPLE PREPARATION We employ the standard Lysis buffer with the cocktail with protease inhibitors (aprotin,leupeptin, etc) and also microcsytin 1 uM, PMSF and beta mercaptoethanol AMOUNT OF PROTEIN LOADED We loaded around 35 micrograms of protein per well ELECTROPHORESIS/GEL CONDITIONS (PAGE-SDS) 10-15 % acrylamide TRANSFER AND BLOCKING CONDITIONS transfer: 1,5 h at 100 v blocking 1 h with TBS plus 0.1% tween and 5% dried deffated milk HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Time of incubation and dilution of the primary antibody

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2004

    Answer

    I'm sorry to hear you've had trouble with ab1371. Thank you for taking the time to fill in the self-help section to contact us, the information you have given me is very useful. I think you have taken all the right steps to get the antibody working and I will arrange a refund for this antibody immediately. As you mentioned, a number of customers have reported similar results in their preparations and we will investigate this further. Once again my sincere apologies,

    Read More

    Abcam Scientific Support

    回复于 Sep 21 2004

    Question

    Attached you will find a piqture of the last Western performed to test the anti-ATF4 (ab1371) using HepG2 cells. (the two first lanes are cell lysate from unstimulated cells, the next two are transfected with LDLR and the last two are tunicamycin stimulated cells). The same protocol as described earlier has been used (4µl antibody /ml blocking buffer). The conclusion is that this antibody bind unspecifically to a lot of bands also in human material. We have no tested this antibody in three concentrations on cell lysate from CHO cells and one time with cell lysate from human HepG2 cells and every time we have got a high amount of unspecific binding to several bands at a level which make the antibody impossible to use. I hope that we finally satisfy AbCams demand for testing and that you will provide us with a new lot of ab1371.

    Read More

    Abcam community

    Verified customer

    Asked on May 12 2004

    Answer

    Thank you for your enquiry and providing more details of your assay. As we understand from your e-mail, the main problem you are experiencing is that you have a lot of non-specific bands. We have done our Western blot analysis with rabbit anti-goat IgG HRP and have only one distinct band at around 40 kDa (using Raji cell lysate). As we have indicated in our previous e-mail, we have not tested this primary antibody in hamster so we do not now if it works in this species. Reading through your protocol we see that you use 5% non-fat dry milk for blocking and a bovine anti-goat IgG conjugated with HRP as secondary antibody. Since the blocking agent is milk and the secondary antibody was raised in bovine, it is probable that there is a cross-reaction. The other alternative is that cow (bovine) and goat - these two species - phylogenetically are not very different from each other and the secondary antibody cross-reacting with endogenous immunoglobulins in the sample For more information about how to choose an antibody, please take a look at the following website: https://www.abcam.com/index.html?pageconfig=tech&mode=choosing Therefore we would suggest testing the secondary antibody alone (run a “secondary control” without the primary antibody) to see if you still have non-specific bands. We would also recommend using either a rabbit or a donkey polyclonal secondary antibody instead of bovine one. For your peace of mind, in case there may be some problem with the primary antibody, certainly we can offer you a replacement vial. If you would like to get it, then please do let us know and confirm your Purchase Order Number or Abcam Order Number. We are looking forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    回复于 May 13 2004

    Question

    As far as I know ATF4 from Chinese Hamster has not been sequenced, but hamster proteins are usually very similar to rat and mouse proteins. Nevertheless, our problems with the strong unspecific bands have noting to do with the similarity of hamster and human proteins. From your answear I couldn't find any solution to that problem. What about providing us with a new lot of the antibody, as stated in the product datasheet: "Quality is important to Abcom. If a product does not perform as described on the datasheet, a replacement or refund will be offered".

    Read More

    Abcam community

    Verified customer

    Asked on May 04 2004

    Answer

    Thank you for your message. As we understand from your e-mail you say that "hamster proteins are usually very similar to rat and mouse proteins". However, we would like to emphasize the fact that we haven't tested this particular antibody on hamster. It is possible that there is some cross-reaction with a 100 kDa protein in hamster that doesn't happen in human. All tested applications are specified on Abcam product datasheets. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

    Read More

    Abcam Scientific Support

    回复于 May 06 2004

    1-10 of 13 Abreviews or Q&A

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