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AB77590

Anti-ASS1抗体

Anti-ASS1 antibody

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(5 Publications)

Goat Polyclonal ASS1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ASS1.

查看别名

ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB77590)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB77590)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab77590 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody (AB77590)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody (AB77590)

IHC image of ab77590 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77590, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry (Intracellular) - Anti-ASS1 antibody (AB77590)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ASS1 antibody (AB77590)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab77590 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB77590)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB77590)

ICC/IF image of ab77590 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween (no animal sera) for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77590 at 10μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human ASS1. The exact immunogen used to generate this antibody is proprietary information.

P00966

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替代产品

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
Biological function summary

Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.

Pathways

ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.

ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
See full target information ASS1
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 2:100318 PubMed34195683

2021

In-depth proteomics analysis of sentinel lymph nodes from individuals with endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soulaimane Aboulouard,Maxence Wisztorski,Marie Duhamel,Philippe Saudemont,Tristan Cardon,Fabrice Narducci,Anne-Sophie Lemaire,Firas Kobeissy,Eric Leblanc,Isabelle Fournier,Michel Salzet

Scientific reports 11:9730 PubMed33958644

2021

Macrophage potentiates the recovery of liver zonation and metabolic function after acute liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Miura,Takashi Hosono,Taiichiro Seki

Cell 182:1606-1622.e23 PubMed32888429

2020

The Human and Mouse Enteric Nervous System at Single-Cell Resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Eugene Drokhlyansky,Christopher S Smillie,Nicholas Van Wittenberghe,Maria Ericsson,Gabriel K Griffin,Gokcen Eraslan,Danielle Dionne,Michael S Cuoco,Max N Goder-Reiser,Tatyana Sharova,Olena Kuksenko,Andrew J Aguirre,Genevieve M Boland,Daniel Graham,Orit Rozenblatt-Rosen,Ramnik J Xavier,Aviv Regev

Hepatology (Baltimore, Md.) 62:417-28 PubMed26011400

2015

Modeling correction of severe urea cycle defects in the growing murine liver using a hybrid recombinant adeno-associated virus/piggyBac transposase gene delivery system.

Applications

Unspecified application

Species

Unspecified reactive species

Sharon C Cunningham,Susan M Siew,Claus V Hallwirth,Christine Bolitho,Natsuki Sasaki,Gagan Garg,Iacovos P Michael,Nicola A Hetherington,Kevin Carpenter,Gustavo de Alencastro,Andras Nagy,Ian E Alexander

Molecular therapy : the journal of the American So 21:1823-31 PubMed23817206

2013

Adeno-associated virus-mediated rescue of neonatal lethality in argininosuccinate synthetase-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cindy Y Kok,Sharon C Cunningham,Kevin H Carpenter,Allison P Dane,Susan M Siew,Grant J Logan,Philip W Kuchel,Ian E Alexander
View all publications
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