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使用敲除细胞株进行验证

Anti-ASS1抗体[2B10] (ab124465)

Submit a review Submit a question References (13)
Western blot - Anti-ASS1 antibody [2B10] (ab124465)
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
  • Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
  • ELISA - Anti-ASS1 antibody [2B10] (ab124465)
  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)

Key features and details

  • Mouse monoclonal [2B10] to ASS1
  • Suitable for: WB, ELISA, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

概述

  • 产品名称

    Anti-ASS1抗体[2B10]
    参阅全部 ASS1 一抗

  • 描述

    小鼠单克隆抗体[2B10] to ASS1

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human, African green monkey

  • 免疫原

    Purified recombinant fragment of Human ASS1, expressed in E. Coli.

  • 阳性对照

    • ICC/IF: HeLa cells. Flow Cyt: Jurkat cells. WB: Human ASS1 recombinant protein; HAP1, A431, Raji, L1210, MOLT4, Jurkat, A549, NIH 3T3, PC12 and COS7 cell lysates.

  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR119162-30 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124465于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/1000.
说明
WB
1/500 - 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/1000.

靶标

  • 通路

    Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.
    Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.

  • 疾病相关

    Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.

  • 序列相似性

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Argininosuccinate synthase 1 antibody
    • Argininosuccinate synthase antibody
    • Argininosuccinate synthetase 1 antibody
    • ASS antibody
    • Ass-1 antibody
    • ass1 antibody
    • ASSA antibody
    • ASSY_HUMAN antibody
    • Citrulline aspartate ligase antibody
    • Citrulline--aspartate ligase antibody
    • CTLN1 antibody
    see all

图片

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ASS1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124465 at 1/1000 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
    Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)
    ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).
  • ELISA - Anti-ASS1 antibody [2B10] (ab124465)
    ELISA - Anti-ASS1 antibody [2B10] (ab124465)
    ELISA using ab124465.
  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    All lanes : Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : L1210 cell lysate
    Lane 4 : MOLT4 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : A549 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate

    Predicted band size: 47 kDa

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Western blot - Anti-ASS1 antibody [2B10] (ab124465)
    Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution + Human ASS1 recombinant protein (aa: 40-236)

    Predicted band size: 47 kDa

文献 (13)

发表研究结果有使用 ab124465？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab124465 被引用在 13 文献中.

  • Ivanova ON  et al. Transcriptome Analysis of Redox Systems and Polyamine Metabolic Pathway in Hepatoma and Non-Tumor Hepatocyte-like Cells. Biomolecules 13:N/A (2023). PubMed: 37189460
  • Missiaen R  et al. GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment. Cell Metab 34:1151-1167.e7 (2022). PubMed: 35839757
  • He Y  et al. Novel Blood Vascular Endothelial Subtype-Specific Markers in Human Skin Unearthed by Single-Cell Transcriptomic Profiling. Cells 11:N/A (2022). PubMed: 35406678
  • Sciacovelli M  et al. Dynamic partitioning of branched-chain amino acids-derived nitrogen supports renal cancer progression. Nat Commun 13:7830 (2022). PubMed: 36539415
  • Soria LR  et al. Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders. EMBO Mol Med 13:e13158 (2021). PubMed: 33369168
View all Publications for this product

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