Anti-Asporin抗体(ab31303)
Key features and details
- Goat polyclonal to Asporin
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Asporin抗体
参阅全部 Asporin 一抗 -
描述
山羊多克隆抗体to Asporin -
宿主
Goat -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Asporin aa 150-250 (internal sequence).
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阳性对照
- WB: Human tonsil, human uterus, mouse and rat skeletal muscle tissue lysates. ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
| ICC |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
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ICC
Use at an assay dependent concentration. |
靶标
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功能
Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. -
组织特异性
Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Co-localizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage. -
疾病相关
Genetic variations in ASPN are associated with susceptibility to osteoarthritis type 3 (OS3) [MIM:607850]; also known as osteoarthritis of knee/hip. Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Note=Susceptibility to osteoarthritis is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is overrepresented relative to the common allele having 13 aspartic acid repeats (D13). The frequency of the D14 allele increases with disease severity. The D14 allele is also overrepresented in individuals with hip osteoarthritis.
Defects in ASPN are a cause of susceptibility to intervertebral disk disease (IDD) [MIM:603932]. A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Note=Susceptibility to intervertebral disk disease, particularly lumbar disk degeneration, is conferred by a triplet repeat expansion polymorphism. ASPN allele having 14 aspartic acid repeats in the N-terminal region of the protein (D14), is associated with the disorder in some populations (PubMed:18304494). -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
结构域
The LRR 5 repeat can inhibit BMP2-induced cytodifferentiation and may be involved in the interaction with BMP2 (By similarity). The repeats LRR 10, LRR 11 and LRR 12 are involved in binding type I collagen. The poly-Asp region is involved in binding calcium. -
翻译后修饰
There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.
The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 54829 Human
- Entrez Gene: 66695 Mouse
- Entrez Gene: 306805 Rat
- Omim: 608135 Human
- SwissProt: Q9BXN1 Human
- SwissProt: Q99MQ4 Mouse
- Unigene: 435655 Human
- Unigene: 383216 Mouse
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别名
- ASPN antibody
- ASPN protein antibody
- ASPN_HUMAN antibody
see all
图片
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Western blot - Anti-Asporin antibody (ab31303)Lanes 1 & 3 & 5 : Anti-Asporin antibody (ab31303) at 0.1 µg/ml
Lane 2 : Anti-Asporin antibody (ab31303) at 0.3 µg/ml
Lane 4 : Anti-Asporin antibody (ab31303) at 1 µg/ml
Lane 1 : Human tonsil tissue lysate
Lane 2 : Human uterus tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Rat skeletal muscle tissue lysate
Lane 5 : Human cerebellum tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa35µg protein in RIPA buffer. Detected by chemiluminescence.
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Immunocytochemistry - Anti-Asporin antibody (ab31303)Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Incubated with ab31303 for 1 hour (10µg/ml) followed by Alexa Fluor® 488 secondary antibody (4µg/ml), showing nuclear membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor® 488 secondary antibody (4µg/ml).
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Western blot - Anti-Asporin antibody (ab31303)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Asporin antibody (ab31303) at 1/1000 dilution
All lanes : Human osteosarcoma cell line whole lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-goat IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% milk for 1 hour at 27°C.
Incubated with the primary antibody diluted in blocking buffer for 12 hours at 4°C.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab31303 被引用在 10 文献中.
- Zhou G et al. ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts. Orthop Surg 15:2423-2434 (2023). PubMed: 37427673
- Chen Y et al. Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis. Theranostics 12:1074-1096 (2022). PubMed: 35154475
- Yin L et al. Identification of a five m6A-relevant mRNAs signature and risk score for the prognostication of gastric cancer. J Gastrointest Oncol 13:2234-2248 (2022). PubMed: 36388685
- Huang C et al. Asporin, an extracellular matrix protein, is a beneficial regulator of cardiac remodeling. Matrix Biol 110:40-59 (2022). PubMed: 35470068
- Wietecha MS et al. Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds. Nat Commun 11:2604 (2020). PubMed: 32451392
- Wang Y et al. Low SCRIB expression in fibroblasts promotes invasion of lung cancer cells. Life Sci 256:117955 (2020). PubMed: 32534038
- Maccarana M et al. Asporin-deficient mice have tougher skin and altered skin glycosaminoglycan content and structure. PLoS One 12:e0184028 (2017). PubMed: 28859141
- Hurng JM et al. Discontinuities in the human bone-PDL-cementum complex. Biomaterials 32:7106-17 (2011). ICC/IF ; Human . PubMed: 21774982
- Duval E et al. Asporin expression is highly regulated in human chondrocytes. Mol Med 17:816-23 (2011). PubMed: 21528154
- Ho SP et al. The biomechanical characteristics of the bone-periodontal ligament-cementum complex. Biomaterials 31:6635-46 (2010). IHC-P ; Human . PubMed: 20541802