重组Anti-Aspartate beta hydroxylase抗体[EPR10351] (ab172475)
![Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.cn/ps/products/172/ab172475/Images/ab172475-194726-anti-aspartate-beta-hydroxylase-antibody-epr10351-western-blot.jpg "Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10351] to Aspartate beta hydroxylase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Aspartate beta hydroxylase抗体[EPR10351]
参阅全部 Aspartate beta hydroxylase 一抗 -
描述
兔单克隆抗体[EPR10351] to Aspartate beta hydroxylase -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Aspartate beta hydroxylase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q12797 -
阳性对照
- HeLa, A549 and HepG2 whole cell lysate (ab7900); Human colon carcinoma and pancreas tissues; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR10351 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172475于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa).
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/250 - 1/500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 86 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
靶标
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相关性
ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor like domains of various proteins. -
细胞定位
Endoplasmic reticulum; endoplasmic reticulum membrane; Single-pass type II membrane protein. -
数据库链接
- Entrez Gene: 444 Human
- Omim: 600582 Human
- SwissProt: Q12797 Human
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别名
- A beta H J J antibody
- AAH antibody
- ASP beta hydroxylase antibody
see all
图片
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Western blot - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)All lanes : Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.cn/ps/products/172/ab172475/Images/ab172475-194728-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.cn/ps/products/172/ab172475/Images/ab172475-194727-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling Aspartate beta hydroxylase with ab172475 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.cn/ps/products/172/ab172475/Images/ab172475-194729-anti-aspartate-beta-hydroxylase-antibody-epr10351-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Immunofluorescent analysis of HeLa cells labeling Aspartate beta hydroxylase with ab172475 at 1/250 dilution (red). DAPI nuclear staining (blue).
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![Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.cn/ps/products/172/ab172475/Images/ab172475-194730-anti-aspartate-beta-hydroxylase-antibody-epr10351-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)Intracellular flow cytometric analysis of permeabilized HeLa cells labeling Aspartate beta hydroxylasewith ab172475at 1/10 dilution (red) compared to a Rabbit IgG (negative) (green).
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![Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)](https://www.abcam.cn/ps/products/172/ab172475/Images/ab172475-6-benefits-of-recombinant-antibodies.png)
Anti-Aspartate beta hydroxylase antibody [EPR10351] (ab172475)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab172475 被引用在 1 文献中.
- Chen X et al. miR-135a acts as a tumor suppressor by targeting ASPH in endometrial cancer. Int J Clin Exp Pathol 12:3384-3389 (2019). PubMed: 31934181