AB189863

Anti-Aspartate Aminotransferase 抗体 - C-terminal

Name: Aspartate Aminotransferase抗体- C-terminal (ab189863)| Abcam中文官网
Brand: Abcam Limited
SKU: AB189863
Price: 4829 CNY
Availability: InStock

Anti-Aspartate Aminotransferase antibody - C-terminal

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(5 Publications)

Rabbit Polyclonal Aspartate Aminotransferase antibody. C-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Aspartate aminotransferase, cytoplasmic aa 200 to C-terminus.

查看别名

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

1 Images

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody - C-terminal (AB189863)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody - C-terminal (ab189863) at 1/500 dilution

Lane 1:

Jurkat cell extract

Lane 2:

293T cell extract

Lane 3:

HepG2 cell extract

Lane 4:

A549 cell extract

Lane 5:

HeLa cell extract

Lane 6:

mouse liver extract

Lane 7:

mouse kidney extract

Lane 8:

mouse heart extract

Predicted band size: 46 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

Recombinant Fragment Protein within Human Aspartate aminotransferase, cytoplasmic aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17174

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反应性数据

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.

Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).

See full target information Aspartate aminotransferase, cytoplasmic

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2088 PubMed40025024

2025

In situ valence-transited arsenic nanosheets for multi-modal therapy of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyue Zheng,Ke Zhang,Jigang Piao,Chaofeng Mu,Xiaowei Xie,Mengying Cheng,Tianxiang Yue,Jiang Sun,Bin Li,Yinghui Wei,Hangsheng Zheng,Lai Jiang,Douae Nihed Habiballah,Fanzhu Li

International journal of urology : official journal of the Japanese Urological Association 28:459-465 PubMed33403726

2021

Age-related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Suo Zou,Takahiro Shimizu,Masaki Yamamoto,Shogo Shimizu,Youichirou Higashi,Takashi Karashima,Motoaki Saito

Journal of molecular and cellular cardiology 138:304-317 PubMed31836543

2019

Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi R Ali,Cole R Michel,Ying H Lin,Timothy A McKinsey,Mark Y Jeong,Amrut V Ambardekar,Joseph C Cleveland,Richard Reisdorph,Nichole Reisdorph,Kathleen C Woulfe,Kristofer S Fritz

Journal of proteome research 18:1513-1531 PubMed30644754

2019

Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi R Ali,Mohammed A Assiri,Peter S Harris,Cole R Michel,Youngho Yun,John O Marentette,Frank K Huynh,David J Orlicky,Colin T Shearn,Laura M Saba,Richard Reisdorph,Nichole Reisdorph,Matthew D Hirschey,Kristofer S Fritz

Cell metabolism 28:817-832.e8 PubMed30244971

2018

Translational and HIF-1α-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Hulea,Simon-Pierre Gravel,Masahiro Morita,Marie Cargnello,Oro Uchenunu,Young Kyuen Im,Camille Lehuédé,Eric H Ma,Matthew Leibovitch,Shannon McLaughlan,Marie-José Blouin,Maxime Parisotto,Vasilios Papavasiliou,Cynthia Lavoie,Ola Larsson,Michael Ohh,Tiago Ferreira,Celia Greenwood,Gaëlle Bridon,Daina Avizonis,Gerardo Ferbeyre,Peter Siegel,Russell G Jones,William Muller,Josie Ursini-Siegel,Julie St-Pierre,Michael Pollak,Ivan Topisirovic

View all publications

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Anti-Aspartate Aminotransferase 抗体 - C-terminal

Anti-Aspartate Aminotransferase antibody - C-terminal

Western blot - Anti-Aspartate Aminotransferase antibody - C-terminal (AB189863)