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重组RabMAb

重组Anti-ASH2L抗体[EPR13107(B)] -核Marker (ab176334)

Submit a review Submit a question References (2)
Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
  • Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13107(B)] to ASH2L - Nuclear Marker
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-ASH2L抗体[EPR13107(B)] -核Marker
    参阅全部 ASH2L 一抗

  • 描述

    兔单克隆抗体[EPR13107(B)] to ASH2L -核Marker

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human ASH2L aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UBL3

  • 阳性对照

    • WB: HeLa, 293T, Jurkat and K562 cell lysates. IHC-P: Human colon, breast and testis tissues, mouse liver, and rat cerebrum tissues. ICC/IF: HeLa and 293T cells. Flow Cyt (intra): HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176334于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/1200.
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/50-1/100

See IHC antigen retrieval protocols.
WB
1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
说明
Flow Cyt (Intra)
1/1200.
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/50-1/100

See IHC antigen retrieval protocols.
WB
1/10000 - 1/50000. Predicted molecular weight: 69 kDa.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.

  • 组织特异性

    Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes.

  • 序列相似性

    Contains 1 B30.2/SPRY domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ASH 2 antibody
      • Ash2 (absent small or homeotic) like antibody
      • Ash2 (absent, small, or homeotic) like (Drosophila) antibody
      • ASH2 antibody
      • ASH2 LIKE antibody
      • ASH2 like protein antibody
      • ASH2-like protein antibody
      • Ash2l antibody
      • ASH2L_HUMAN antibody
      • ASH2L1 antibody
      • ASH2L2 antibody
      • Bre 2 antibody
      • Bre2 antibody
      • Discs 2-like antibody
      • Drosophila absent, small, or homeotic antibody
      • Drosophila ASH2-like antibody
      • Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
      see all

    图片

    • Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      All lanes : Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334) at 1/10000 dilution (Purified)

      Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 69 kDa
      Observed band size: 65,80 kDa why is the actual band size different from the predicted?



      ab176334 recoginzes 3 isoforms of ASH2L

    • Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling ASH2L with purified ab176334 at 1/50 dilution (7.78 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling ASH2L with purified ab176334 at 1/8000 dilution (0.05 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Western blot - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      All lanes : Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334) at 1/10000 dilution ((unpurified))

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : Jurkat cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 69 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunofluorescent analysis of HeLa labeling ASH2L with ab176334 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with unpurified ab176334 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with unpurifiedab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with unpurified ab176334 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)
      Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker (ab176334)

    文献 (2)

    发表研究结果有使用 ab176334？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab176334 被引用在 2 文献中.

    • Rao RA  et al. KMT1 family methyltransferases regulate heterochromatin-nuclear periphery tethering via histone and non-histone protein methylation. EMBO Rep 20:N/A (2019). PubMed: 30858340
    • Jung Y  et al. An epigenetic mechanism mediates developmental nicotine effects on neuronal structure and behavior. Nat Neurosci 19:905-14 (2016). PubMed: 27239938

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