Anti-ASH1L抗体[ASH5H03] (ab50981)
![Western blot - Anti-ASH1L antibody [ASH5H03] (ab50981)](https://www.abcam.cn/ps/products/50/ab50981/Images/ab50981-62786-anti-ash1l-antibody-ash5h03-western-blot.gif "Western blot - Anti-ASH1L antibody [ASH5H03] (ab50981)")
Key features and details
- Mouse monoclonal [ASH5H03] to ASH1L
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-ASH1L抗体[ASH5H03] -
描述
小鼠单克隆抗体[ASH5H03] to ASH1L -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment
预测可用于: Human
-
免疫原
Recombinant fragment corresponding to Human ASH1L aa 1611-1728.
Sequence:IGSCRVSNPNSSGRKKLTDSPGLFSAQDTSLNRLHRKESLPSNERAVQTL AGSQPTSDKPSQRPSESTNCSPTRKRSSSESTSSTVNGVPSRSPRLVASG DDSVDSLLQRMVQNEDQE
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, 0.03% Potassium phosphate, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab50981 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum -
克隆
单克隆 -
克隆编号
ASH5H03 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50981于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
WB: Use at an assay dependent dilution. Predicted molecular weight: 330 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Histone methyltransferase. Probably methylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation. -
组织特异性
Widely expressed, with highest level in brain, heart and kidney. -
序列相似性
Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
Contains 3 A.T hook DNA-binding domains.
Contains 1 AWS domain.
Contains 1 BAH domain.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 post-SET domain.
Contains 1 SET domain. -
细胞定位
Nucleus. Cell junction > tight junction. Chromosome. The relevance of tight junction localization is however unclear. - Information by UniProt
-
数据库链接
- Entrez Gene: 55870 Human
- Omim: 607999 Human
- SwissProt: Q9NR48 Human
- Unigene: 491060 Human
-
别名
- Absent small and homeotic disks protein 1 homolog antibody
- Absent Small or Homeotic Like antibody
- Absent Small or Homeotic Like antibody
see all
图片
-
Western blot - Anti-ASH1L antibody [ASH5H03] (ab50981)Anti-ASH1L antibody [ASH5H03] (ab50981) + Recombinant ASH1L (immunogen)
Predicted band size: 330 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
数据表及文件
-
Datasheet download
文献 (3)
ab50981 被引用在 3 文献中.
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Atta H et al. Mutant MMP-9 and HGF gene transfer enhance resolution of CCl4-induced liver fibrosis in rats: role of ASH1 and EZH2 methyltransferases repression. PLoS One 9:e112384 (2014). Dot blot . PubMed: 25380300
- Perugorria MJ et al. Histone methyltransferase ASH1 orchestrates fibrogenic gene transcription during myofibroblast transdifferentiation. Hepatology 56:1129-39 (2012). WB . PubMed: 22488473
