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重组RabMAb

重组Anti-ASF1A抗体[EPR28755-1] - BSA and Azide free (ab317503)

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Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
  • Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
  • Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
  • Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
  • Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
  • Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28755-1] to ASF1A - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-ASF1A抗体[EPR28755-1] - BSA and Azide free
    参阅全部 ASF1A 一抗

  • 描述

    兔单克隆抗体[EPR28755-1] to ASF1A - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Human
    不与反应: Rat

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HCT 116, SW480, Jurkat, NIH/3T3, WEHI-231 and His-tagged human ASF1A recombinant protein. Flow Cyt (Intra): HeLa and NIH/3T3 cells.

  • 常规说明

    ab317503 is the carrier-free version of ab317502.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317503于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

  • 组织特异性

    Ubiquitously expressed.

  • 序列相似性

    Belongs to the ASF1 family.

  • 翻译后修饰

    Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Anti silencing function 1A antibody
      • Anti-silencing function protein 1 homolog A antibody
      • ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
      • ASF1 anti silencing function 1 homolog A antibody
      • asf1a antibody
      • ASF1A_HUMAN antibody
      • CCG1 interacting factor A antibody
      • CCG1-interacting factor A antibody
      • CGI 98 antibody
      • CIA antibody
      • hAsf1 antibody
      • hAsf1a antibody
      • hCIA antibody
      • Histone chaperone ASF1A antibody
      • HSPC146 antibody
      see all

    图片

    • Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      All lanes : Anti-ASF1A antibody [EPR28755-1] (ab317502) at 1/1000 dilution

      Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
      Lane 2 : HeLa transfected with siRNA specifically targeting ASF1A whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 19 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317502, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      All lanes : Anti-ASF1A antibody [EPR28755-1] (ab317502) at 1/1000 dilution

      Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
      Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 5 : WEHI-231 (mouse b cell lymphoma b lymphocyte ) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 19 kDa why is the actual band size different from the predicted?


      Exposure time: 158 seconds


      This data was developed using ab317502, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      Western blot - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      All lanes : Anti-ASF1A antibody [EPR28755-1] (ab317502) at 1/1000 dilution

      Lane 1 : His-tagged human ASF1A recombinant protein 10 ng
      Lane 2 : His-tagged human ASF1B recombinant protein 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 19 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This data was developed using ab317502, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody does not cross-react with human ASF1B.

      In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade  (ab213204) staining at 1/5000 dilution.

    • Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)

      This data was developed using ab317502, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling ASF1A with ab317502 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)

      This data was developed using ab317502, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ASF1A with ab317502 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)
      Anti-ASF1A antibody [EPR28755-1] - BSA and Azide free (ab317503)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317503？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317503 尚未被引用在任何文献中。

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