重组Anti-ASF1A抗体[EPR28755-1] (ab317502)
![Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)](https://www.abcam.cn/ps/products/317/ab317502/Images/ab317502-539015-anti-asf1a-antibody-epr28755-1-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-transfected-with-scrambled-sirna-control-whole-cell-lysate-20-ug.jpg "Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28755-1] to ASF1A
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-ASF1A抗体[EPR28755-1]
参阅全部 ASF1A 一抗 -
描述
兔单克隆抗体[EPR28755-1] to ASF1A -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
不与反应: Rat -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HCT 116, SW480, Jurkat, NIH/3T3, WEHI-231 and His-tagged human ASF1A recombinant protein. Flow Cyt (Intra): HeLa and NIH/3T3 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28755-1 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| WB |
1/1000.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. |
靶标
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功能
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the ASF1 family. -
翻译后修饰
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 25842 Human
- Entrez Gene: 66403 Mouse
- Omim: 609189 Human
- SwissProt: Q9Y294 Human
- SwissProt: Q9CQE6 Mouse
- Unigene: 292316 Human
- Unigene: 272989 Mouse
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别名
- Anti silencing function 1A antibody
- Anti-silencing function protein 1 homolog A antibody
- ASF1 anti silencing function 1 homolog A (S. cerevisiae) antibody
see all
图片
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Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)All lanes : Anti-ASF1A antibody [EPR28755-1] (ab317502) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting ASF1A whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)](https://www.abcam.cn/ps/products/317/ab317502/Images/ab317502-539041-anti-asf1a-antibody-epr28755-1-western-blot-hct-116-human-colorectal-carcinoma-epithelial-cell-whole-cell-lysate30-ug.jpg)
Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)All lanes : Anti-ASF1A antibody [EPR28755-1] (ab317502) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 2 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 5 : WEHI-231 (mouse b cell lymphoma b lymphocyte ) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 158 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)](https://www.abcam.cn/ps/products/317/ab317502/Images/ab317502-539042-anti-asf1a-antibody-epr28755-1-western-blot-his-tagged-human-asf1a-recombinant-protein-10-ng.jpg)
Western blot - Anti-ASF1A antibody [EPR28755-1] (ab317502)All lanes : Anti-ASF1A antibody [EPR28755-1] (ab317502) at 1/1000 dilution
Lane 1 : His-tagged human ASF1A recombinant protein 10 ng
Lane 2 : His-tagged human ASF1B recombinant protein 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human ASF1B.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
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![Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] (ab317502)](https://www.abcam.cn/ps/products/317/ab317502/Images/ab317502-2-anti-asf1a-antibody-epr28755-1-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] (ab317502)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling ASF1A with ab317502 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] (ab317502)](https://www.abcam.cn/ps/products/317/ab317502/Images/ab317502-3-anti-asf1a-antibody-epr28755-1-flow-cytometry-nih3t3.jpg)
Flow Cytometry (Intracellular) - Anti-ASF1A antibody [EPR28755-1] (ab317502)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ASF1A with ab317502 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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![Anti-ASF1A antibody [EPR28755-1] (ab317502)](https://www.abcam.cn/ps/products/317/ab317502/Images/ab317502-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-ASF1A antibody [EPR28755-1] (ab317502)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317502 尚未被引用在任何文献中。