AB204348

Anti-ASCT-1抗体

Name: ASCT-1抗体 (ab204348)| Abcam中文官网
Brand: Abcam Limited
SKU: AB204348
Price: 4606 CNY
Availability: InStock

Anti-ASCT-1 antibody

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(1 Publication)

Rabbit Polyclonal ASCT-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC1A4 aa 450 to C-terminus.

查看别名

ASCT1, SATT, SLC1A4, Neutral amino acid transporter A, Alanine/serine/cysteine/threonine transporter 1, Solute carrier family 1 member 4, ASCT-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ASCT-1 antibody (AB204348)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASCT-1 antibody (AB204348)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling ASCT-1 with ab204348 at 4 μg/ml (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCT-1 antibody (AB204348)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCT-1 antibody (AB204348)

Immunohistochemical analysis of paraffin-embedded Human oral mucosa tissue labeling ASCT-1 with ab204348 at 1/20 dilution.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human SLC1A4 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P43007

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Alanine Serine Cysteine-preferring Transporter 1 (ASCT-1) also known as SLC1A4 functions as a Na+-dependent transport protein for neutral amino acids including alanine serine and cysteine. It has a molecular mass of approximately 56 kDa. ASCT-1 expresses on the plasma membrane and is found in the brain and the retina. Its role in the transport of certain amino acids is critical for maintaining normal cellular functions and metabolic processes.

Biological function summary

ASCT-1 transports amino acids which contributes to cellular nutrition and neurotransmitter cycling. It does not function as part of a larger protein complex but rather as a standalone transporter. ASCT-1 plays a significant role in neuronal health by regulating amino acids in the synaptic cleft which impacts neurotransmitter synthesis and recycling essential for proper neural communication.

Pathways

ASCT-1 is actively involved in the glutamate cycle which maintains neurotransmitter homeostasis in the brain. Its activity facilitates the uptake of serine used in the synthesis of D-serine an important co-agonist in NMDA receptor pathways. Both the ASCT-2 transporter and SLC1A3 also part of the glutamate transporter family share related functions in these pathways ensuring the synaptic glutamate levels are regulated effectively.

ASCT-1 mutations or dysregulation have links to neurodevelopmental disorders including intellectual disability and epilepsy. Alterations in its function disrupt amino acid transport which can affect neurotransmitter cycling contributing to these conditions. Additionally ASCT-1 shares a connection with SLC1A5 in these disorders where overlapping functions in amino acid transport may exacerbate the symptoms when mutated or dysfunctional.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Sodium-coupled antiporter of neutral amino acids. In a tri-substrate transport cycle, exchanges neutral amino acids between the extracellular and intracellular compartments, coupled to the inward cotransport of at least one sodium ion (PubMed : 24808181, PubMed : 27272177, PubMed : 34630942, PubMed : 8910405). Exchanges neutral amino acids such as L- and D-serine, L-threonine, L-asparagine and L-alanine in a bidirectional way (PubMed : 14502423, PubMed : 26041762, PubMed : 27272177, PubMed : 34630942, PubMed : 8101838, PubMed : 8340364, PubMed : 8910405). Involved in homeostasis of D-serine, a coagonist of synaptic NMDA receptors. In astrocytes at excitatory synapses, mediates electrogenic D-serine influx coupled to L-serine efflux and L-serine shuttling to neurons for de novo D-serine synthesis (By similarity). Displays sodium- and amino acid-dependent but uncoupled channel-like anion conductance with a preference SCN(-) > NO3(-) > I(-) > Cl(-) (PubMed : 8910405).

See full target information SLC1A4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36769360

2023

Haploinsufficiency Impairs PI3K/AKT/mTOR/Autophagy Pathway in Human Pluripotent Stem Cells Derived Neural Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junwen Wang,Ziyi Wang,Limeng Dai,Xintong Zhu,Xingying Guan,Junyi Wang,Jia Li,Mao Zhang,Yun Bai,Hong Guo

View all publications

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Anti-ASCT-1抗体

Anti-ASCT-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ASCT-1 antibody (AB204348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASCT-1 antibody (AB204348)