AB150368

重组Anti-ASCC2抗体[EPR9773]

Anti-ASCC2 antibody [EPR9773]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal ASCC2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

查看别名

ASC1P100, RQT3, ASCC2, Activating signal cointegrator 1 complex subunit 2, ASC-1 complex subunit p100, Trip4 complex subunit p100

3 Images

Unknown

Western blot - Anti-ASCC2 antibody [EPR9773] (AB150368)

All lanes:

Western blot - Anti-ASCC2 antibody [EPR9773] (ab150368) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 86 kDa

false

Lab

Western blot - Anti-ASCC2 antibody [EPR9773] (AB150368)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ASCC2 antibody [EPR9773] (ab150368) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Observed band size: 86 kDa

false

Lab

Western blot - Anti-ASCC2 antibody [EPR9773] (AB150368)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ASCC2 antibody [EPR9773] (ab150368) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Lane 3:

RAW264.7 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 86 kDa

Observed band size: 86 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-ASCC2 antibody [EPR9773] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9773

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as ASCC2 also referred to as Activating Signal Cointegrator 2 Complex Subunit 2 functions mechanically as a regulator in cellular processes. ASCC2 has a molecular mass of approximately 95 kDa. This protein plays a role in transcriptional regulation and is expressed in various tissues including the brain and liver. By interacting with other proteins ASCC2 acts in the modification of gene expression impacting cell behavior and function.

Biological function summary

ASCC2 forms part of the ASCC complex contributing to DNA repair processes and the regulation of transcription. The complex holds an important position in maintaining genomic integrity which is necessary for proper cellular function. Scientists recognize ASCC2 for its role in modulating transcriptional responses to environmental stressors by interacting with specific transcription factors. Its involvement in these processes highlights the importance of ASCC2 in cellular adaptability and resilience.

Pathways

ASCC2 interacts with important biological routes such as the transcription regulation pathway and the DNA damage response pathway. Within these pathways ASCC2 partners with proteins like ASCC1 and ASCC3 working in coordination to regulate cellular stress responses and repair DNA. This interaction highlights ASCC2’s pivotal role in maintaining cellular homeostasis and adaptability to stress conditions.

ASCC2 holds relevance in conditions such as cancer and neurodegenerative disorders. Its interactions can influence pathogenesis and progression in these diseases. The protein links to other figures implicated in these processes such as ASCC1 with ASCC2's expression levels often correlating with disease states. Understanding its mechanisms and interdependencies highlights the potential for ASCC2 as a target in therapeutic strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-binding protein involved in DNA repair and rescue of stalled ribosomes (PubMed : 29144457, PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Plays a role in DNA damage repair as component of the ASCC complex (PubMed : 29144457). Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed : 29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed : 12077347). Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). Specifically recognizes and binds RPS20/uS10 ubiquitinated by ZNF598, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes (PubMed : 36302773).

See full target information ASCC2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:78 PubMed38844873

2024

Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Liu,Mengbi Zhang,Lu Zeng,Yanhong Lai,Songzhao Wu,Xiaoyan Su

Autophagy 18:2985-3003 PubMed35414338

2022

LC3 subfamily in cardiolipin-mediated mitophagy: a comparison of the LC3A, LC3B and LC3C homologs.

Applications

Unspecified application

Species

Unspecified reactive species

Marina N Iriondo,Asier Etxaniz,Yaiza R Varela,Uxue Ballesteros,Javier H Hervás,L Ruth Montes,Félix M Goñi,Alicia Alonso

View all publications

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重组Anti-ASCC2抗体[EPR9773]