Anti-ASC2/ASCI抗体(ab47092)
Key features and details
- Rabbit polyclonal to ASC2/ASCI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-ASC2/ASCI抗体 -
描述
兔多克隆抗体to ASC2/ASCI -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human ASC2/ASCI.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Associates with apoptosis-associated specklike protein containing a CARD domain (ASC) and modulates its ability to collaborate with pyrin and cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS. -
组织特异性
Predominantly expressed in monocytes, macrophages and granulocytes. -
序列相似性
Contains 1 DAPIN domain. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 260434 Human
- SwissProt: Q8WXC3 Human
- Unigene: 58314 Human
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别名
- PAAD domain only protein antibody
- PAAD-only protein 1 antibody
- POP1 antibody
see all
图片
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Western blot - Anti-ASC2/ASCI antibody (ab47092)All lanes : Anti-ASC2/ASCI antibody (ab47092) at 1/2000 dilution
Lane 1 : HEK-293 cells transiently transfected with plasmid encoding ASC2/ASCI
Lane 2 : HEK-293 cells transiently transfected with plasmid encoding POP1
Lane 3 : HEK-293 cells transiently transfected with a negative control plasmid
Predicted band size: 10 kDa
Observed band size: 10 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC2/ASCI antibody (ab47092)Immunhistochemical analysis of ASC2/ASCI/POP1 expression in mouse spleen using ab47092 at 1:2000. Left and Right images: spleen at low and high magnification, respectively. ASC2/ASCI/POP1 staining is seen in the granulocytes, whereas the lymph and megakaryocytes are negative.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC2/ASCI antibody (ab47092)Immunhistochemical analysis of ASC2/ASCI/POP1 expression in mouse using ab47092 at 1:2000. A, prostate tissue from a male mouse prostatitis. B, kidney from a mouse with pyelonephritis. A1 and B1, high magnification of A and B, respectively. ASC2/ASCI/POP1 staining is seen in the granulocytes
数据表及文件
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Datasheet download
文献 (14)
ab47092 被引用在 14 文献中.
- Dong D et al. VX-765 Alleviates β-Amyloid Deposition and Secondary Degeneration in the Ipsilateral Hippocampus and Ameliorates Cognitive Decline after Focal Cortical Infarction in Rats. J Mol Neurosci 72:2389-2397 (2022). PubMed: 36441377
- Zhou W et al. MicroRNA-1929-3p participates in murine cytomegalovirus-induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation. Int J Mol Med 47:719-731 (2021). PubMed: 33416142
- Xiang N et al. Gardnerella vaginalis induces NLRP3 inflammasome-mediated pyroptosis in macrophages and THP-1 monocytes. Exp Ther Med 22:1174 (2021). PubMed: 34504619
- Wang G et al. LPS-induced macrophage HMGB1-loaded extracellular vesicles trigger hepatocyte pyroptosis by activating the NLRP3 inflammasome. Cell Death Discov 7:337 (2021). PubMed: 34743181
- Liang Y et al. Inhibition of Caspase-1 Ameliorates Ischemia-Associated Blood-Brain Barrier Dysfunction and Integrity by Suppressing Pyroptosis Activation. Front Cell Neurosci 14:540669 (2020). PubMed: 33584203
