AB70627

Anti-ASC1 抗体

Name: ASC1抗体 (ab70627)| Abcam中文官网
Brand: Abcam Limited
SKU: AB70627
Price: 4514 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-ASC1 antibody

(2 Reviews)

(18 Publications)

Rabbit Polyclonal ASC1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human TRIP4 aa 200-300.

查看别名

RQT4, TRIP4, Activating signal cointegrator 1, ASC-1, Thyroid receptor-interacting protein 4, TR-interacting protein 4, TRIP-4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (AB70627)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (AB70627)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling ASC1 with ab70627 at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-ASC1 antibody (AB70627)

ASC1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70627at 3 μg per reaction. Western blot was performed on the immunoprecipitate using ab70627 at 1 μg/mL.

Lane 1 : ab70627 IP in HeLa whole cell lysate.

Lane 2 : ab70627 IP in HeLa whole cell lysate.

Lane 3 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-ASC1 antibody (ab70627)

Predicted band size: 66 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ASC1 antibody (AB70627)

All lanes:

Western blot - Anti-ASC1 antibody (ab70627) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 66 kDa

false

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, IHC-P, WB

applications

免疫原

Synthetic Peptide within Human TRIP4 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q15650

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ASC1 protein also known as ASC-1 is a multifunctional protein involved in several cellular processes. It has a mass of approximately 41 kDa and is primarily expressed in the nucleus of cells. Some studies highlight its presence in diverse tissues indicating a potential broad involvement in different cellular activities. While the protein itself is known common names like ASCONE procedure refer to specialized contexts or nomenclature systems related to ASC1.

Biological function summary

ASC1 plays a significant role in the regulation of transcription and acts as a coactivator of nuclear receptors. It forms part of a larger transcription regulatory complex partnering with other coactivators and transcriptional machinery to enhance gene expression. Its interaction capacity signifies its importance in modulating gene expression patterns thereby affecting cellular physiology at the transcriptional regulation level.

Pathways

ASC1 engages primarily with the nuclear receptor signaling pathways and transcription regulation pathways. Within these pathways it interacts with other proteins like steroid receptor coactivators to modulate various responses to hormonal signals. This integration into complex pathways highlights ASC1's importance in maintaining cellular homeostasis and response to external signals.

ASC1 is linked to cancers particularly hormone-responsive cancers like breast and prostate cancer. Its interaction with nuclear receptors like estrogen receptor and androgen receptor connects ASC1 to the regulation of these cancers' progression. Moreover mutations or dysregulation of ASC1 can contribute to the aberrant expression of genes that promote tumorigenicity. Understanding ASC1 interactions with other coactivators can provide insight into potential therapeutic targets for these cancers.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription (PubMed : 10454579, PubMed : 25219498). May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions (PubMed : 10454579, PubMed : 25219498). Plays a role in thyroid hormone receptor and estrogen receptor transactivation (PubMed : 10454579, PubMed : 25219498). Also involved in androgen receptor transactivation (By similarity). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1 (PubMed : 12077347). Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B (PubMed : 12077347). May play a role in the development of neuromuscular junction (PubMed : 26924529). May play a role in late myogenic differentiation (By similarity). Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed : 32099016, PubMed : 32579943, PubMed : 36302773).

See full target information TRIP4

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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:159 PubMed39333859

2024

Role of caspase-11 non-canonical inflammasomes in retinal ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wan,Jiayu Li,Jialei Pu,Jing Yang,Cheng Pei,Yun Qi

NPJ Parkinson's disease 10:156 PubMed39147828

2024

rTMS improves dysphagia by inhibiting NLRP3 inflammasome activation and caspase-1 dependent pyroptosis in PD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Peiling Huang,Ziman Zhu,Wenshan Li,Rong Zhang,Yijia Chi,Weijun Gong

Clinical and experimental pharmacology & physiology 51:e13910 PubMed39073215

2024

Oxycodone attenuates lipopolysaccharide-induced myocardial injury by inhibiting inflammation, oxidation and pyroptosis via Nrf2/HO-1 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanting Wang,Wei Feng,Shaona Li,Cuicui Liu,Lili Jia,Pei Wang,Linlin Li,Hongyin Du,Wenli Yu

Anais da Academia Brasileira de Ciencias 96:e20230053 PubMed38451595

2024

Bone-marrow mononuclear cells and acellular human amniotic membrane improve global cardiac function without inhibition of the NLRP3 Inflammasome in a rat model of heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Aline L Takejima,Paulo André B Machado-Júnior,Gustavo G Blume,Rossana Baggio Simeoni,Julio Cesar Francisco,Murilo S Tonial,Luis Felipe B Marqueze,Lucia Noronha,Marcia Olandoski,Eltyeb Abdelwahid,Katherine A T DE Carvalho,Ricardo A Pinho,Luiz César Guarita-Souza

Chemical biology & drug design 103:e14436 PubMed38395608

2024

Isoliquiritigenin attenuates neuroinflammation after subarachnoid hemorrhage through inhibition of NF-κB-mediated NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Wenfei,Tao Xiang,Chen Chen,Tao Yang,Tao Yun,Chen Zhibiao,Zhang Ge

Molecular medicine (Cambridge, Mass.) 29:44 PubMed37013491

2023

MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Zhao,Wei Hua,Qi Liu,Yueying Wang,Zhiyi Liu,Sinan Jin,Benshuai Wang,Yuxin Pang,Jiping Qi,Yuejia Song

BMC cardiovascular disorders 23:10 PubMed36627567

2023

Uric acid promotes myocardial infarction injury via activating pyrin domain-containing 3 inflammasome and reactive oxygen species/transient receptor potential melastatin 2/Capathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Wu,Ruozhu Dai,Min Wang,Chengbo Chen

Molecular vision 28:500-506 PubMed37089701

2022

Epac1 and PKA agonists inhibit ROS to reduce NLRP3 inflammasome proteins in retinal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Youde Jiang,Jena J Steinle

Molecular medicine reports 25: PubMed34850960

2021

FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Ouyang,Xiaoqin Zhao,Rongping Zhang,Jing Yang,Siyin Li,Daihua Deng

American journal of translational research 13:11384-11398 PubMed34786066

2021

LncRNA H19 activates cell pyroptosis via the miR-22-3p/NLRP3 axis in pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyang Sun,Shun Mao,Wei Ji

View all publications

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Anti-ASC1 抗体

Anti-ASC1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (AB70627)

Immunoprecipitation - Anti-ASC1 antibody (AB70627)

Western blot - Anti-ASC1 antibody (AB70627)