Anti-ASC1抗体(ab127537)
Key features and details
- Rabbit polyclonal to ASC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-ASC1抗体
参阅全部 ASC1 一抗 -
描述
兔多克隆抗体to ASC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
-
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-272 of Human ASC1 (Q15650).
-
阳性对照
- A549 and HCT116 whole cell lysates; Human colon carcinoma tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab127537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 66 kDa.
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 66 kDa. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Transcription coactivator of nuclear receptors which functions in conjunction with CBP-p300 and SRC-1 and may play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function. -
细胞定位
Nucleus. Cytoplasm. Cytoplasmic under conditions of serum deprivation. - Information by UniProt
-
数据库链接
- Entrez Gene: 533430 Cow
- Entrez Gene: 9325 Human
- Entrez Gene: 56404 Mouse
- Entrez Gene: 315769 Rat
- Omim: 604501 Human
- SwissProt: Q15650 Human
- SwissProt: Q9QXN3 Mouse
- Unigene: 500340 Human
see all -
别名
- Activating signal cointegrator 1 antibody
- ASC 1 antibody
- ASC-1 antibody
see all
图片
-
Western blot - Anti-ASC1 antibody (ab127537)Anti-ASC1 antibody (ab127537) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 66 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASC1 antibody (ab127537)ab127537, at 1/250, staining ASC1 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab127537 被引用在 5 文献中.
- Wu DM et al. Autophagy Induced by Micheliolide Alleviates Acute Irradiation-Induced Intestinal Injury via Inhibition of the NLRP3 Inflammasome. Front Pharmacol 12:773150 (2021). PubMed: 35115927
- Yu Y et al. Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling. Front Immunol 11:593368 (2020). PubMed: 33362775
- Qian W et al. Astragaloside IV protects endothelial progenitor cells from the damage of ox-LDL via the LOX-1/NLRP3 inflammasome pathway. Drug Des Devel Ther 13:2579-2589 (2019). PubMed: 31440038
- Zhu J et al. Protective effects of Erigeron breviscapus Hand.- Mazz. (EBHM) extract in retinal neurodegeneration models. Mol Vis 24:315-325 (2018). PubMed: 29769797
- Qi Y et al. Retinal ischemia/reperfusion injury is mediated by Toll-like receptor 4 activation of NLRP3 inflammasomes. Invest Ophthalmol Vis Sci 55:5466-75 (2014). PubMed: 25097240