Anti-ASAH2抗体(ab63804)
Key features and details
- Rabbit polyclonal to ASAH2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ASAH2抗体
参阅全部 ASAH2 一抗 -
描述
兔多克隆抗体to ASAH2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide surrounding amino acid 741 of human ASAH2
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阳性对照
- Jurkat cell lysate.
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3828 ASAH2 Antibody. 3828-100 is the same size as the 100 µg size of ab63804.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63804于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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| 说明 |
|---|
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
靶标
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功能
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. -
组织特异性
Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B. -
序列相似性
Belongs to the neutral ceramidase family. -
翻译后修饰
N-glycosylated. Required for enzyme activity.
O-glycosylated. Required to retain it as a type II membrane protein at the cell surface.
Phosphorylated. May prevent ubiquitination and subsequent degradation.
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid. -
细胞定位
Cell membrane. The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N-terminus, which may explain this localization which probably does not exist in vivo. - Information by UniProt
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数据库链接
- Entrez Gene: 56624 Human
- Entrez Gene: 653308 Human
- Omim: 611202 Human
- SwissProt: Q9NR71 Human
- Unigene: 512645 Human
- Unigene: 710005 Human
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别名
- Acylsphingosine deacylase 2 antibody
- AI585898 antibody
- ASAH2 antibody
see all
图片
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Western blot - Anti-ASAH2 antibody (ab63804)All lanes : Anti-ASAH2 antibody (ab63804) at 4 µg/ml
All lanes : Jurkat cell lysate
Predicted band size: 86 kDa
Observed band size: 86 kDa
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab63804 被引用在 1 文献中.
- Zhu Q et al. Low-dose cytokine-induced neutral ceramidase secretion from INS-1 cells via exosomes and its anti-apoptotic effect. FEBS J 281:2861-70 (2014). PubMed: 24798654