AB252990

Anti-ASAH2抗体

Name: ASAH2抗体 (ab252990)| Abcam中文官网
Brand: Abcam Limited
SKU: AB252990
Price: 5791 CNY
Availability: InStock

Anti-ASAH2 antibody

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(1 Publication)

Rabbit Polyclonal ASAH2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASAH2 aa 1-100.

查看别名

HNAC1, ASAH2, Neutral ceramidase, N-CDase, NCDase, Acylsphingosine deacylase 2, BCDase, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, hCD

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ASAH2 antibody (AB252990)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ASAH2 antibody (AB252990)

PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for ASAH2 (green) using ab252990 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAH2 antibody (AB252990)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAH2 antibody (AB252990)

Paraffin-embedded human duodenum tissue stained for ASAH2 using ab252990 at 1/1000 dilution in immunohistochemical analysis.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ASAH2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NR71

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ASAH2 also known as N-acylsphingosine amidohydrolase 2 is an important enzyme responsible for the hydrolysis of ceramides into sphingosine and free fatty acids. It has a molecular mass of approximately 85 kDa. ASAH2 is expressed in many tissues with prominent expression observed in the small intestine and brain. Its activity influences cell membrane integrity and intercellular communication which plays an essential role in the normal functioning of cells.

Biological function summary

ASAH2 regulates the levels of ceramide and sphingosine both of which are important lipid signaling molecules. This enzyme exists within cells as part of a complex that maintains lipid homeostasis impacting numerous biological processes through ceramide metabolism. The balance between ceramide and sphingosine signaling is important for cell survival proliferation and apoptosis highlighting the importance of ASAH2 in these cellular dynamics.

Pathways

ASAH2 involves itself in the sphingolipid metabolism pathway which is significant for signal transduction and cellular communication. ASAH2 works closely with proteins like sphingosine kinase which further metabolizes sphingosine into sphingosine-1-phosphate (S1P) a lipid mediator with diverse functions. This pathway plays essential roles in inflammation immune response and cell growth demonstrating the interconnected nature of ASAH2's activity with other critical proteins.

ASAH2 has associations with metabolic disorders and neurodegenerative diseases. Elevated ceramide levels due to ASAH2 dysfunction relate to disorders such as Gaucher disease characterized by abnormal lipid storage and may influence neurodegenerative conditions like Alzheimer's disease. The enzyme interacts with proteins like acid ceramidase where imbalances in these enzymes lead to disruptions in lipid metabolism and contribute to disease pathology making ASAH2 a potential target for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed : 10781606, PubMed : 16229686, PubMed : 26190575). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed : 15946935, PubMed : 19345744, PubMed : 24798654). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed : 11278489, PubMed : 17475390). Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed : 16061940). Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (By similarity). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (By similarity).

See full target information ASAH2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:836041 PubMed35281088

2022

iTRAQ-Based Proteome Profiling of Differentially Expressed Proteins in Insulin-Resistant Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yan,Bei Xie,Ye Tian,Li Huang,Shuli Zou,Zhiheng Peng,Zhuan Liu,Linjing Li

View all publications

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Anti-ASAH2抗体

Anti-ASAH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ASAH2 antibody (AB252990)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASAH2 antibody (AB252990)