Anti-AS160 (phospho T642)抗体(ab65753)
Key features and details
- Sheep polyclonal to AS160 (phospho T642)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-AS160 (phospho T642)抗体
参阅全部 AS160 一抗 -
描述
羊多克隆抗体to AS160 (phospho T642) -
宿主
Sheep -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human AS160 aa 600-700 conjugated to keyhole limpet haemocyanin.
Database link: O60343 -
阳性对照
- WB: HEK-293 cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65753于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa.
|
| 说明 |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa. |
靶标
-
功能
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. -
组织特异性
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis. -
序列相似性
Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain. -
翻译后修饰
Phosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. -
细胞定位
Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 9882 Human
- Omim: 612465 Human
- SwissProt: O60343 Human
- Unigene: 210891 Human
-
别名
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
see all
图片
-
Western blot - Anti-AS160 (phospho T642) antibody (ab65753)All lanes : Anti-AS160 (phospho T642) antibody (ab65753) at 2 µg/ml
Lane 1 : AS160 wild type (WT)
Lane 2 : AS160 wild type (WT) with (+) 1 µM insulin treatment for 15 minutes
Lane 3 : S588A mutant (S588A)
Lane 4 : S588A mutant (S588A) with (+) 1 µM insulin treatment for 15 minutes
Lane 5 : T642A mutant (T642A)
Lane 6 : T642A mutant (T642A) with (+) 1 µM insulin treatment for 15 minutes
Predicted band size: 147 kDaWestern blot analysis of immunoprecipitated Flagtagged AS160 wild type (WT), S588A mutant (S588A), and T642A mutant (T642A) over-expressed transiently in HEK-293 cells, basal (-) or with (+) 1 µM insulin treatment for 15 minutes.
数据表及文件
-
Datasheet download
文献 (8)
ab65753 被引用在 8 文献中.
- Actis Dato V et al. Targeting cholesteryl ester accumulation in the heart improves cardiac insulin response. Biomed Pharmacother 152:113270 (2022). PubMed: 35709652
- Ramos PA et al. Insulin-Stimulated Muscle Glucose Uptake and Insulin Signaling in Lean and Obese Humans. J Clin Endocrinol Metab 106:e1631-e1646 (2021). PubMed: 33382888
- Actis Dato V et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892
- Lu R et al. Protective role of Astragaloside IV in chronic glomerulonephritis by activating autophagy through PI3K/AKT/AS160 pathway. Phytother Res 34:3236-3248 (2020). PubMed: 32726508
- Yang GS et al. mTOR is involved in stroke-induced seizures and the anti-seizure effect of mild hypothermia. Mol Med Rep 17:5821-5829 (2018). PubMed: 29484389
- Krag TO et al. Differential glucose metabolism in mice and humans affected by McArdle disease. Am J Physiol Regul Integr Comp Physiol 311:R307-14 (2016). PubMed: 27280431
- Inazuka F et al. Muscle-specific Knock-out of NUAK Family SNF1-like Kinase 1 (NUAK1) Prevents High Fat Diet-induced Glucose Intolerance. J Biol Chem 287:16379-89 (2012). WB ; Mouse . PubMed: 22418434
- Schweitzer GG et al. Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation. J Appl Physiol 113:1852-61 (2012). WB ; Rat . PubMed: 22936728
