重组Anti-Artemin抗体[EPR9763] (ab178434)
![Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)](https://www.abcam.cn/ps/products/178/ab178434/Images/ab178434-7-anti-artemin-antibody-epr9763-immunoprecipitation-pc12-rat.jpg "Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9763] to Artemin
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Artemin抗体[EPR9763]
参阅全部 Artemin 一抗 -
描述
兔单克隆抗体[EPR9763] to Artemin -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Artemin aa 150 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q5T4W7 -
阳性对照
- WB: T-47D, NIH/3T3 and PC-12 whole cell lysate. Mouse and rat brain lysate; PC-12 whole cell lysate. IP: PC-12 whole cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9763 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178434于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
|
IP
1/10 - 1/100. |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. -
组织特异性
Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain. -
序列相似性
Belongs to the TGF-beta family. GDNF subfamily. -
发展阶段
Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 9048 Human
- Entrez Gene: 11876 Mouse
- Entrez Gene: 362572 Rat
- Omim: 603886 Human
- SwissProt: Q5T4W7 Human
- SwissProt: Q9Z0L2 Mouse
- SwissProt: Q6AYE8 Rat
- Unigene: 632404 Human
see all -
别名
- Artemin antibody
- Artn antibody
- ARTN_HUMAN antibody
see all
图片
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Immunoprecipitation - Anti-Artemin antibody [EPR9763] (ab178434)Purified ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input): PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+): ab178434 + PC-12 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa -
![Western blot - Anti-Artemin antibody [EPR9763] (ab178434)](https://www.abcam.cn/ps/products/178/ab178434/Images/ab178434-449364-anti-artemin-antibody-epr9763-westernblot-human-mouse-rat.jpg)
Western blot - Anti-Artemin antibody [EPR9763] (ab178434)All lanes : Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution
Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDa -
![Anti-Artemin antibody [EPR9763] (ab178434)](https://www.abcam.cn/ps/products/178/ab178434/Images/ab178434-5-benefits-of-recombinant-antibodies.png)
Anti-Artemin antibody [EPR9763] (ab178434)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab178434 被引用在 6 文献中.
- Iwasaki T et al. Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs. Int J Mol Sci 24:N/A (2023). PubMed: 37958856
- Zhu M et al. Artemin Promotes the Migration and Invasion of Cervical Cancer Cells through AKT/mTORC1 Signaling. J Oncol 2022:3332485 (2022). PubMed: 36471885
- Li TJ et al. Human splenic TER cells: A relevant prognostic factor acting via the artemin-GFRα3-ERK pathway in pancreatic ductal adenocarcinoma. Int J Cancer 148:1756-1767 (2021). PubMed: 33236361
- Hou Y et al. Radiotherapy and immunotherapy converge on elimination of tumor-promoting erythroid progenitor cells through adaptive immunity. Sci Transl Med 13:N/A (2021). PubMed: 33627484
- Song Z et al. Role of artemin in non-small cell lung cancer. Thorac Cancer 9:555-562 (2018). PubMed: 29575549
- Wang J et al. Artemin regulates CXCR4 expression to induce migration and invasion in pancreatic cancer cells through activation of NF-?B signaling. Exp Cell Res 365:12-23 (2018). Human . PubMed: 29453972