AB70800

Anti-ARTD10 抗体

Name: ARTD10抗体 (ab70800)| Abcam中文官网
Brand: Abcam Limited
SKU: AB70800
Price: 4606 CNY
Availability: InStock

Anti-ARTD10 antibody

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(3 Publications)

Rabbit Polyclonal ARTD10 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PARP10 aa 300-350.

查看别名

Protein mono-ADP-ribosyltransferase PARP10, ADP-ribosyltransferase diphtheria toxin-like 10, Poly [ADP-ribose] polymerase 10, ARTD10, PARP-10, PARP10

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (AB70800)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (AB70800)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labeling ARTD10 with ab70800 at 1/1000 (1μg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-ARTD10 antibody (AB70800)

1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ARTD10 antibody (ab70800)

Predicted band size: 123 kDa

false

Unknown

Western blot - Anti-ARTD10 antibody (AB70800)

All lanes:

Western blot - Anti-ARTD10 antibody (ab70800) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 123 kDa

Observed band size: 123 kDa,60 kDa,70 kDa,90 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, IP

applications

免疫原

Synthetic Peptide within Human PARP10 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q53GL7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein ARTD10 also known as PARP10 is a member of the poly(ADP-ribose) polymerase (PARP) family. It exhibits a mass of approximately 100 kDa. ARTD10 acts mechanically as mono-ADP-ribosyltransferase which modifies target proteins by adding ADP-ribose units. This specific post-translational modification controls protein function and interactions. ARTD10 expression occurs mostly in the cytoplasm and nucleus across different cell types indicating its versatile functional roles.

Biological function summary

ARTD10 influences several cellular processes including transcriptional regulation and cell cycle progression. It functions independently without being part of a stable protein complex but interacts with other nuclear and cytoplasmic proteins to exert its role. This protein is involved in modulating cellular responses to DNA damage and stress impacting cellular homeostasis and metabolism. Its interaction patterns highlight its significance in cellular regulatory mechanisms.

Pathways

ARTD10 is an active participant in DNA repair and stress response pathways. It is closely linked with the NF-kB signaling pathway where it modulates inflammation and cell survival outcomes. ARTD10 also connects with proteins like PARP1 sharing a broader role in the maintenance of genomic integrity and response to DNA insults. These pathways underpin the critical mechanisms by which cells manage and adapt to damages and maintain cellular viability.

ARTD10's dysregulation associates with certain cancers and inflammatory conditions. Aberrant activity of ARTD10 impacts cellular proliferation and apoptosis linking it with tumorigenesis. Inflammation-related disorders also see a correlation with altered ARTD10 activity. This protein remains related to key proteins like NF-kB in understanding its role in these diseases as they collectively influence inflammatory and tumor progression pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate and aspartate residues on target proteins (PubMed : 18851833, PubMed : 23332125, PubMed : 23474714, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 18851833). Catalyzes mono-ADP-ribosylation of GSK3B, leading to negatively regulate GSK3B kinase activity (PubMed : 23332125). Involved in translesion DNA synthesis in response to DNA damage via its interaction with PCNA (PubMed : 24695737).

See full target information PARP10

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and molecular hepatology 31:841-865 PubMed39905842

2025

Glutamate dehydrogenase 1-dependent α-ketoglutarate promotes hepatitis B virus transcription by modulating histone methylations on the covalently closed circular DNA minichromosome.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Tao Cheng,Wei-Xian Chen,Hai-Jun Deng,Xin He,Hui Zhang,Ming Tan,Hai-Bo Yu,Zhen-Zhen Zhang,Ji-Hua Ren,Min-Li Yang,Da-Peng Zhang,Zhi-Hong Li,Juan Chen

Nature communications 15:6197 PubMed39043663

2024

PARP10 promotes the repair of nascent strand DNA gaps through RAD18 mediated translesion synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jude B Khatib,Ashna Dhoonmoon,George-Lucian Moldovan,Claudia M Nicolae

Frontiers in genetics 13:1035638 PubMed36313419

2022

Depletion of PARP10 inhibits the growth and metastatic potential of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zihui Zhou,Bing Wei,Yu Liu,Tian Liu,Sien Zeng,Jinfeng Gan,Guangying Qi

View all publications

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Anti-ARTD10 抗体

Anti-ARTD10 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTD10 antibody (AB70800)

Immunoprecipitation - Anti-ARTD10 antibody (AB70800)

Western blot - Anti-ARTD10 antibody (AB70800)