AB192870

重组Anti-ART3抗体[EPR7220(2)] - C-terminal

Anti-ART3 antibody [EPR7220(2)] - C-terminal

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal ART3 antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

查看别名

TMART, ART3, Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)--arginine ADP-ribosyltransferase 3, ARTC3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - C-terminal (AB192870)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - C-terminal (AB192870)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as secondary. Counterstain : Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - C-terminal (AB192870)

Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody

Note : The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).

All lanes:

Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)

Predicted band size: 36 kDa,44 kDa

Observed band size: 36 kDa

false

Supplier Data

Western blot - Anti-ART3 antibody [EPR7220(2)] - C-terminal (AB192870)

All lanes:

Western blot - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution

All lanes:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 41 kDa,44 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-ART3 antibody [EPR7220(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7220(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ART3 also known as ADP-ribosyltransferase 3 is an enzyme with a molecular mass of approximately 41 kDa. It belongs to the family of ARTs (ADP-ribosyltransferases) which facilitate the transfer of ADP-ribose from NAD+ to target proteins modifying their function. ART3 is mainly expressed on the surface of cells with higher expression observed in testicular tissues. Its distribution suggests a specialized role in certain physiological processes.

Biological function summary

ART3 modifies protein functions by adding ADP-ribose groups a process important for cellular signaling and protein interactions. While it is not as deeply investigated as other ART family members ART3 still plays significant roles in certain biological contexts. It operates independently rather than being part of a larger complex. Current research explores its involvement in sperm maturation and other reproductive functions especially due to its elevated expression in testicular tissue.

Pathways

ART3's actions integrate into the wider cellular signaling network influenced by ADP-ribosylation. This modification impacts pathways such as cell communication and possibly apoptosis although detailed mapping in these contexts remains developing. ART3 shares mechanistic similarities with proteins such as ART1 and ART5 which also perform ADP-ribosylation and align with changes in protein activity and signaling dynamics.

ART3 has potential implications in reproductive health and certain cancers. Altered ART3 expression or function may link to testes-related disorders or contribute to cellular oncogenesis. It connects with other proteins like ART1 whose dysregulation has been studied in various cancers. Understanding ART3's contributions could aid in developing targeted treatments or diagnostics related to its roles in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information ART3

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Chemical & pharmaceutical bulletin 73:896-906 PubMed40993069

2025

A Melittin-Derived Peptide with Improved Cytosolic Delivery Efficiency through Caveolae- and Actin-Mediated Endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshimasa Kawaguchi,Naoki Tamemoto,Yusuke Uehata,Yusuke Miyazaki,Wataru Shinoda,Shiroh Futaki

Cell death & disease 12:191 PubMed33602903

2021

c-MYC-induced long noncoding RNA MEG3 aggravates kidney ischemia-reperfusion injury through activating mitophagy by upregulation of RTKN to trigger the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dajun Liu,Ying Liu,Xiaotong Zheng,Naiquan Liu

View all publications

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重组Anti-ART3抗体[EPR7220(2)] - C-terminal