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AB316159

重组Anti-ART1抗体[EPR28754-56]

Anti-ART1 antibody [EPR28754-56]

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Rabbit Recombinant Monoclonal ART1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

查看别名

CD296, GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1, ADP-ribosyltransferase C2 and C3 toxin-like 1, Mono(ADP-ribosyl)transferase 1, ARTC1, ART1

4 Images
Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 12070318).

The identity of the higher MW band at approximately 70 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (ab316159) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 40 µg

Lane 2:

Human spleen tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37 kDa,36 kDa

true

Exposure time: 81s

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 12070318).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 10 seconds, lane 2 : 180 seconds

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (ab316159) at 1/1000 dilution

Lane 1:

Rat skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (ab316159) at 1/1000 dilution

Lane 1:

Untreated C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 2:

C2C12 differentiation to muscle for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)
  • WB

Supplier Data

Western blot - Anti-ART1 antibody [EPR28754-56] (AB316159)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 12070318), brain (PMID : 12070318).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ART1 antibody [EPR28754-56] (ab316159) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

  • Carrier free

    Anti-ART1 antibody [EPR28754-56] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR28754-56

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse, Rat

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB108403

Anti-CD38 antibody [EPR4106]

20ul selling size|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

  • 0
  • 1
Primary Antibodies

AB192870

Anti-ART3 antibody [EPR7220(2)] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - C-terminal (AB192870)

  • 0
  • 0
Primary Antibodies

AB314504

Anti-SQSTM1 / p62 antibody [RM1079] - Autophagosome Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [RM1079] - Autophagosome Marker (AB314504)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Conditional Ambient
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ART1 also known as ADP-ribosyltransferase 1 functions mechanically as an enzyme that adds ADP-ribose groups onto arginine residues on target proteins. This post-translational modification plays a role in regulating the function of the substrate proteins. ART1 has a molecular mass approximately around 37 kDa. Expression of ART1 is notable in skeletal muscle and also in various epithelial tissues. The presence of ART1 in these tissues suggests its involvement in several physiological processes.
Biological function summary

ART1 contributes to cellular signaling and substrate protein regulation by altering protein interactions. ART1 does not function as part of a significant large protein complex. However its enzymatic activity impacts the modification and activity of other proteins influencing various cellular responses and processes. These modifications can result in dynamic changes within cellular signaling pathways affecting cellular functions like growth cell cycle and immune response.

Pathways

ART1 participates in pathways related to NAD+ metabolism and ADP-ribosylation. Through these pathways ART1 influences the function of proteins such as integrins and stress response proteins. ART1 by modulating protein activity can affect broader biological processes like cell adhesion and response to cellular stress linked to the pathways it participates in.

ART1 relates to conditions such as muscle dystrophy and inflammatory diseases. In muscle dystrophies ART1-mediated ADP-ribosylation affects muscle cell integrity and signaling impacting disease progression. Additionally its interaction with immune proteins like interferon-induced proteins places ART1 as a potential player in inflammatory processes suggesting its involvement in inflammatory diseases. Through these interactions ART1 might contribute to inflammation regulation and tissue integrity in disease contexts.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Has ADP-ribosyltransferase activity toward GLP1R.
See full target information GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
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Abcam Product Promise

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详情请参阅我们的条款与条件。

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