AB192999

Anti-ARS2抗体- N-terminal

Name: ARS2抗体- N-terminal (ab192999)| Abcam中文官网
Brand: Abcam Limited
SKU: AB192999
Price: 4606 CNY
Availability: InStock

Anti-ARS2 antibody - N-terminal

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(4 Publications)

Rabbit Polyclonal ARS2 antibody. N-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SRRT aa 1-50.

查看别名

ARS2, ASR2, SRRT, Serrate RNA effector molecule homolog, Arsenite-resistance protein 2

2 Images

Supplier Data

Immunoprecipitation - Anti-ARS2 antibody - N-terminal (AB192999)

Detection of ARS2 in immunoprecipitates of Jurkat whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192999 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-ARS2 antibody - N-terminal (ab192999)

Predicted band size: 101 kDa

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Supplier Data

Western blot - Anti-ARS2 antibody - N-terminal (AB192999)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ARS2 antibody - N-terminal (ab192999) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 101 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human SRRT aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BXP5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab192999 was affinity purified using an epitope specific to ARS2 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ARS2 also known as SRRT (serrate RNA effector molecule homolog) is a human protein encoded by the SRRT gene. ARS2 is a nuclear protein with an approximate molecular weight of 96 kDa. It is highly expressed in tissues such as the brain lung and liver. Inside the nucleus ARS2 interacts with various RNA-processing complexes binding to nascent RNA transcripts. The primary role of ARS2 centers around RNA metabolism where it manages and organizes the maturation of small RNAs.

Biological function summary

This protein assists in the capping processing and export of several RNA types including pre-miRNAs and other short-length RNAs. ARS2 forms part of a larger protein complex interacting with the Cap Binding Complex (CBC) and Integrator complex which helps regulate RNA stability and processing. Through these interactions ARS2 facilitates the proper maturation and quality control of RNA which is essential for maintaining cellular function and gene expression regulation.

Pathways

The activity of this protein notably aligns with the mRNA processing pathway and RNA surveillance pathway. Within these pathways ARS2 plays a role by participating in the post-transcriptional regulation that is interlinked with proteins like CBC and the nuclear RNA exosome complex. These collaborations enable tight control over RNA homeostasis ensuring that cells can adapt and respond to various cellular signals and stressors by modifying gene expression profiles.

Abnormalities in ARS2 function associate with neural development issues and cancer. In the context of neural development improper ARS2 activity affects RNA processing linking it to neurodevelopmental disorders where the faulty RNA regulation disrupts normal neural gene expression. Its role in cancer relates to unwanted proliferation stemming from misregulated RNA and altered gene expression patterns. Proteins connected to these diseases through ARS2 include DGCR8 (DiGeorge syndrome critical region gene 8) in neurodevelopment and Drosha in various cancers for their shared importance in RNA processing pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self-renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity).

See full target information SRRT

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 94: PubMed32376621

2020

Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Julio C Ruiz,Anne M Devlin,Jiwoong Kim,Nicholas K Conrad

Nature communications 11:2412 PubMed32415063

2020

Conserved protein Pir2 mediates gene repression through cryptic introns in lncRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Gobi Thillainadesan,Hua Xiao,Sahana Holla,Jothy Dhakshnamoorthy,Lisa M Miller Jenkins,David Wheeler,Shiv I S Grewal

Nature communications 11:1063 PubMed32102997

2020

The role of Mediator and Little Elongation Complex in transcription termination.

Applications

Unspecified application

Species

Unspecified reactive species

Hidehisa Takahashi,Amol Ranjan,Shiyuan Chen,Hidefumi Suzuki,Mio Shibata,Tomonori Hirose,Hiroko Hirose,Kazunori Sasaki,Ryota Abe,Kai Chen,Yanfeng He,Ying Zhang,Ichigaku Takigawa,Tadasuke Tsukiyama,Masashi Watanabe,Satoshi Fujii,Midori Iida,Junichi Yamamoto,Yuki Yamaguchi,Yutaka Suzuki,Masaki Matsumoto,Keiichi I Nakayama,Michael P Washburn,Anita Saraf,Laurence Florens,Shigeo Sato,Chieri Tomomori-Sato,Ronald C Conaway,Joan W Conaway,Shigetsugu Hatakeyama

Oncology reports 40:3313-3322 PubMed30542699

2018

Knockdown of arsenic resistance protein 2 inhibits human glioblastoma cell proliferation through the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Xue Ke,Yi Pang,Kuijun Chen,Dunke Zhang,Feng Wang,Shunqin Zhu,Jingxin Mao,Xiaosong Hu,Guanghui Zhang,Hongjuan Cui

View all publications

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Anti-ARS2抗体- N-terminal

Anti-ARS2 antibody - N-terminal

Immunoprecipitation - Anti-ARS2 antibody - N-terminal (AB192999)

Western blot - Anti-ARS2 antibody - N-terminal (AB192999)