重组Anti-ARPC5/p16 ARC抗体[EP1551Y] (ab51243)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1551Y] to ARPC5/p16 ARC
- Suitable for: ICC/IF, IP, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ARPC5/p16 ARC抗体[EP1551Y]
参阅全部 ARPC5/p16 ARC 一抗 -
描述
兔单克隆抗体[EP1551Y] to ARPC5/p16 ARC -
宿主
Rabbit -
特异性
This antibody is specific to the p16 ARC protein, database link: O15511. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.
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经测试应用
适用于: ICC/IF, IP, WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal, mouse and rat brain tissue lysates and HeLa, HepG2, SKBR-3 and MCF-7 cell lysates. IHC-P: Human uterus adenocarcinoma and spleen tissues. ICC/IF: Neuro-2a cells. IP: Human fetal brain tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1551Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/50 - 1/100.
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IP |
1/20 - 1/70.
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WB | (5) |
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. We have obtained results that indicate IHC-P is unsuitable for mouse and rat species. |
说明 |
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ICC/IF
1/50 - 1/100. |
IP
1/20 - 1/70. |
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. We have obtained results that indicate IHC-P is unsuitable for mouse and rat species. |
靶标
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功能
Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. -
序列相似性
Belongs to the ARPC5 family. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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数据库链接
- Entrez Gene: 10092 Human
- Entrez Gene: 67771 Mouse
- Entrez Gene: 360854 Rat
- Omim: 604227 Human
- SwissProt: O15511 Human
- SwissProt: Q9CPW4 Mouse
- SwissProt: Q4KLF8 Rat
- Unigene: 518609 Human
see all -
别名
- Actin related protein 2/3 complex subunit 5 (16 kD) antibody
- Actin related protein 2/3 complex subunit 5 antibody
- Actin related protein 2/3 complex, subunit 5 16kDa antibody
see all
图片
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: p16 ARC knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab51243 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab51243 was shown to specifically react with p16 ARC when p16 ARC knockout samples were used. Wild-type and p16 ARC knockout samples were subjected to SDS-PAGE. Ab51243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.
Lane 1 (input): Human fetal brain tissue lysate (10µg)
Lane 2 (+): ab51243 + human fetal brain tissue lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51243 in human fetal brain tissue lysate.
For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : SKBR-3 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
All lanes : Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution (purified)
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
All lanes : Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution (unpurified)
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 16 kDaBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab51243 被引用在 9 文献中.
- Wang Y et al. 27-Hydroxycholesterol-Induced Dysregulation of Cholesterol Metabolism Impairs Learning and Memory Ability in ApoE ε4 Transgenic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 36232940
- Indra I et al. Sensing Actin Dynamics through Adherens Junctions. Cell Rep 30:2820-2833.e3 (2020). PubMed: 32101754
- Wang Y et al. 27-Hydroxycholesterol Alters Synaptic Structural and Functional Plasticity in Hippocampal Neuronal Cultures. J Neuropathol Exp Neurol 78:238-247 (2019). PubMed: 30753597
- Choi J et al. Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer Sci 110:3788-3801 (2019). PubMed: 31571309
- Moriya Y et al. Tumor suppressive microRNA-133a regulates novel molecular networks in lung squamous cell carcinoma. J Hum Genet 57:38-45 (2012). PubMed: 22089643
- Kinoshita T et al. Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma. Int J Oncol 40:1770-8 (2012). PubMed: 22378351
- Chang YF et al. Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation. Proc Natl Acad Sci U S A 109:5750-5 (2012). ICC/IF ; Mouse . PubMed: 22447776
- Hubert T et al. Actin and Arp2/3 localize at the centrosome of interphase cells. Biochem Biophys Res Commun 404:153-8 (2011). ICC/IF ; Human . PubMed: 21108927
- Schmidt S et al. Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption. J Cell Biol 192:883-97 (2011). ICC/IF ; Mouse . PubMed: 21357746