Anti-ARPC4抗体(ab80502)
Key features and details
- Goat polyclonal to ARPC4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ARPC4抗体
参阅全部 ARPC4 一抗 -
描述
山羊多克隆抗体to ARPC4 -
宿主
Goat -
特异性
ab80502 is expected to recognise isoform a (NP_005709.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide:
C-ERHNKPEVEVR
, corresponding to internal sequence amino acids 31 - 41 of Human ARPC4 (NP_005709.1) -
阳性对照
- Human lymph node lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab80502 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80502于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 20 kDa.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 20 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. -
序列相似性
Belongs to the ARPC4 family. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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数据库链接
- Entrez Gene: 10093 Human
- Entrez Gene: 68089 Mouse
- Entrez Gene: 297518 Rat
- Omim: 604226 Human
- SwissProt: P59998 Human
- SwissProt: P59999 Mouse
- Unigene: 323342 Human
- Unigene: 289306 Mouse
see all -
别名
- Actin related protein 2/3 complex subunit 4 antibody
- Actin related protein 2/3 complex subunit 4 20 kD antibody
- Actin related protein 2/3 complex subunit 4 20kDa antibody
see all
图片
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Western blot - Anti-ARPC4 antibody (ab80502)Anti-ARPC4 antibody (ab80502) at 0.03 µg/ml + human lymph node lysate (in RIPA buffer) at 35 µg
Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab80502 被引用在 1 文献中.
- Fu X et al. Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons. J Neurosci 33:709-21 (2013). PubMed: 23303949
