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AB80502

Anti-ARPC4抗体

Anti-ARPC4 antibody

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(1 Publication)

Goat Polyclonal ARPC4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARPC4 aa 1-50.

查看别名

ARC20, ARPC4, Actin-related protein 2/3 complex subunit 4, Arp2/3 complex 20 kDa subunit, p20-ARC

1 Images
Western blot - Anti-ARPC4 antibody (AB80502)
  • WB

Unknown

Western blot - Anti-ARPC4 antibody (AB80502)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ARPC4 antibody (ab80502) at 0.03 µg/mL

All lanes:

human lymph node lysate (in RIPA buffer) at 35 µg

Predicted band size: 20 kDa,42 kDa

Observed band size: 19 kDa,41 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Synthetic Peptide within Human ARPC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P59998

特异性

ab80502 is expected to recognise isoform a (NP_005709.1).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
ab80502 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The ARPC4 protein also known as Actin-Related Protein 2/3 Complex Subunit 4 plays a significant role in the actin nucleation process. It is a component of the ARP2/3 complex which is essential for the formation of branched actin filament networks. ARPC4 has a molecular weight of approximately 20 kDa. It is ubiquitously expressed across various tissues indicating its fundamental role in cellular mechanisms.
Biological function summary

ARPC4 contributes to the dynamic remodeling of the actin cytoskeleton. It is an integral part of the ARP2/3 complex a multi-protein structure that facilitates actin filament branching promoting cell movement and shape changes. This complex is critical for processes like cell migration phagocytosis and intracellular trafficking. By aiding in these activities ARPC4 influences cellular responses to environmental signals and stress.

Pathways

Proteins related to cellular movement and structure rely significantly on actin network regulation. ARPC4 through the ARP2/3 complex participates in the regulation of the WAVE regulatory complex (WRC) and is involved in the actin-related pathways that include Rac1 and N-WASP. These pathways are key to transmitting signals that control cytoskeletal reorganization and influence cell polarity movement and division.

Aberrations in the actin filament network including those involving ARPC4 can contribute to pathological conditions. Research suggests that disruptions in ARPC4 function are linked to cancer metastasis due to its role in cell motility. Additionally ARPC4 has connections with neurological disorders through its interaction with proteins like N-WASP and Rac1 which are known to influence neural cell signaling and structure. Understanding the role of ARPC4 in these diseases may offer potential therapeutic targets.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed : 9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed : 9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information ARPC4
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 33:709-21 PubMed23303949

2013

Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Fu,Kristy J Brown,Chan Choo Yap,Bettina Winckler,Jyoti K Jaiswal,Judy S Liu
View all publications
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