Anti-ARPC2抗体(ab11798)
Key features and details
- Goat polyclonal to ARPC2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ARPC2抗体
参阅全部 ARPC2 一抗 -
描述
山羊多克隆抗体to ARPC2 -
宿主
Goat -
特异性
This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601. -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rat, Zebrafish
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免疫原
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
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阳性对照
- WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11798于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.
A 1 hour primary incubation at room temperature is recommended for this product. |
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| ICC/IF |
Use a concentration of 10 µg/ml.
PubMed ID: 22021442 |
| 说明 |
|---|
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IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa. A 1 hour primary incubation at room temperature is recommended for this product. |
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ICC/IF
Use a concentration of 10 µg/ml. PubMed ID: 22021442 |
靶标
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功能
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. -
序列相似性
Belongs to the ARPC2 family. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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数据库链接
- Entrez Gene: 10109 Human
- Entrez Gene: 301511 Rat
- Entrez Gene: 327068 Zebrafish
- Omim: 604224 Human
- SwissProt: O15144 Human
- SwissProt: P85970 Rat
- Unigene: 529303 Human
- Unigene: 93317 Rat
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别名
- Actin related protein 2/3 complex subunit 2 34kDa antibody
- Actin related protein 2/3 complex subunit 2 antibody
- Actin-related protein 2/3 complex subunit 2 antibody
see all
图片
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Western blot - Anti-ARPC2 antibody (ab11798)Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Observed band size: 34-35 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6). -
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab11798 被引用在 5 文献中.
- Xing H et al. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Sci Rep 12:22474 (2022). PubMed: 36577792
- Cheng Z et al. ARPC2 promotes breast cancer proliferation and metastasis. Oncol Rep 41:3189-3200 (2019). PubMed: 31002363
- Xia P et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). PubMed: 25225459
- Chauhan BK et al. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108:18289-94 (2011). ICC/IF ; Mouse . PubMed: 22021442
- Li Y et al. The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails. Eur J Cell Biol 87:893-904 (2008). PubMed: 18707793