Anti-ARPC1A抗体(ab133160)
Key features and details
- Goat polyclonal to ARPC1A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ARPC1A抗体 -
描述
山羊多克隆抗体to ARPC1A -
宿主
Goat -
特异性
This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Pig, Zebrafish -
免疫原
Synthetic peptide corresponding to Human ARPC1A aa 356-368 (C terminal) (Cysteine residue).
Sequence:C-DFKTLESSIQGLR
Database link: NP_006400.2 -
阳性对照
- WB: HeLa cell lysate and mouse and rat brain lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133160于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. -
序列相似性
Belongs to the WD repeat ARPC1 family.
Contains 6 WD repeats. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 508402 Cow
- Entrez Gene: 10552 Human
- Entrez Gene: 56443 Mouse
- Entrez Gene: 100174963 Pig
- Entrez Gene: 81824 Rat
- Entrez Gene: 394243 Zebrafish
- Omim: 604220 Human
- SwissProt: Q1JP79 Cow
see all -
别名
- Actin binding protein (Schizosaccharomyces pombe sop2 like) antibody
- Actin related protein 2/3 complex subunit 1A 41kDa antibody
- Actin-related protein 2/3 complex subunit 1A antibody
see all
图片
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All lanes : Anti-ARPC1A antibody (ab133160) at 0.1 µg/ml
Lane 1 : Mouse brain lysate (in RIPA buffer)
Lane 2 : Adult rat brain lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour.
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Anti-ARPC1A antibody (ab133160) at 0.3 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 42 kDaPrimary incubation for 1 hour.
数据表及文件
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Datasheet download
文献 (1)
ab133160 被引用在 1 文献中.
- Fäßler F et al. ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning. Sci Adv 9:eadd6495 (2023). PubMed: 36662867