Anti-Arp2抗体[FMS96] (ab49674)
Key features and details
- Mouse monoclonal [FMS96] to Arp2
- Suitable for: WB, ICC/IF, Indirect ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
概述
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产品名称
Anti-Arp2抗体[FMS96]
参阅全部 Arp2 一抗 -
描述
小鼠单克隆抗体[FMS96] to Arp2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Indirect ELISAmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human -
免疫原
Recombinant full length protein corresponding to Human Arp2.
Database link: P61160 -
阳性对照
- WB: C2C12 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
FMS96 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Indirect ELISA |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 10 µg/ml. |
Indirect ELISA
Use at an assay dependent concentration. |
靶标
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功能
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
序列相似性
Belongs to the actin family. ARP2 subfamily. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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数据库链接
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
- SwissProt: Q5M7U6 Rat
- Unigene: 643727 Human
see all -
别名
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
图片
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C2C12 cells were incubated with ab49674 at 10 µg/ml followed by Goat Anti Mouse IgG Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.
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Immunocytochemistry/ Immunofluorescence - Anti-Arp2 antibody [FMS96] (ab49674)Image from Aggarwal A et al., PLoS Pathog. 2012 Jun;8(6):e1002762. Epub 2012 Jun 7. Fig 4.; doi:10.1371/journal.ppat.1002762; June 7, 2012, PLoS Pathog 8(6): e1002762.Immunofluorescence analysis of dendritic cells infected with HIV, staining Arp2 (blue) with ab49674 at 5 mg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab49674 被引用在 15 文献中.
- Johnson JL et al. Differential dysregulation of granule subsets in WASH-deficient neutrophil leukocytes resulting in inflammation. Nat Commun 13:5529 (2022). PubMed: 36130971
- Saelens JW et al. An ancestral mycobacterial effector promotes dissemination of infection. Cell 185:4507-4525.e18 (2022). PubMed: 36356582
- González-Martín A et al. c-Abl regulates a synaptic plasticity-related transcriptional program involved in memory and learning. Prog Neurobiol 205:102122 (2021). PubMed: 34284000
- Kutsch M et al. Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions. EMBO J 39:e104926 (2020). PubMed: 32510692
- Plessner M et al. Centrosomal Actin Assembly Is Required for Proper Mitotic Spindle Formation and Chromosome Congression. iScience 15:274-281 (2019). PubMed: 31096079