重组Anti-Arp2抗体[EPR7980] (ab128934)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7980] to Arp2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Arp2抗体[EPR7980]
参阅全部 Arp2 一抗 -
描述
兔单克隆抗体[EPR7980] to Arp2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human uterus tissue; Jurkat, Huvec and MCF7 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 7.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7980 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128934于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. -
序列相似性
Belongs to the actin family. ARP2 subfamily. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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数据库链接
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
- SwissProt: Q5M7U6 Rat
- Unigene: 643727 Human
see all -
别名
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
图片
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All lanes : Anti-Arp2 antibody [EPR7980] (ab128934) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arp2 antibody [EPR7980] (ab128934)
ab128934, at a 1/100 dilution, staining Arp2 in paraffin-embedded human uterus tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab128934 被引用在 9 文献中.
- Arnould C et al. Chromatin compartmentalization regulates the response to DNA damage. Nature 623:183-192 (2023). PubMed: 37853125
- Bogucka-Janczi K et al. ERK3/MAPK6 dictates CDC42/RAC1 activity and ARP2/3-dependent actin polymerization. Elife 12:N/A (2023). PubMed: 37057894
- Nunes-Santos CJ et al. Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling. Nat Commun 14:3708 (2023). PubMed: 37349293
- Zou YJ et al. RAB14 GTPase is essential for actin-based asymmetric division during mouse oocyte maturation. Cell Prolif 54:e13104 (2021). PubMed: 34323331
- Bolger-Munro M et al. Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation. Elife 8:N/A (2019). PubMed: 31157616
- Miller HE et al. Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth. PLoS Pathog 14:e1007005 (2018). PubMed: 29668757
- Braun DA et al. Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly. Nat Genet 49:1529-1538 (2017). PubMed: 28805828
- Kahr WH et al. Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease. Nat Commun 8:14816 (2017). PubMed: 28368018
- He SW et al. SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes. Cell Cycle 16:2272-2281 (2017). PubMed: 28933599