Anti-ARP1抗体(ab11009)
Key features and details
- Rabbit polyclonal to ARP1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ARP1抗体
参阅全部 ARP1 一抗 -
描述
兔多克隆抗体to ARP1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Dog
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免疫原
Synthetic peptide corresponding to Human ARP1 aa 222-240 conjugated to keyhole limpet haemocyanin.
Sequence:ACYLSINPQKDETLETEKAQ
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常规说明
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11009于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| WB | (4) |
| 说明 |
|---|
For indirect immunofluorescence, a minimum working antibody dilution of 1:100 is recommended using mouse fibroblasts NIH3T cell.
WB: 1/1000 (recommended using a rat brain cytosol extract and a whole cell extract of the human epidermoid carcinoma A431 cell line). Predicted molecular weight: 45 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome. -
序列相似性
Belongs to the actin family. ARP1 subfamily. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 403791 Dog
- Entrez Gene: 10121 Human
- Entrez Gene: 294010 Rat
- Omim: 605143 Human
- SwissProt: P61162 Dog
- SwissProt: P61163 Human
- SwissProt: P85515 Rat
- Unigene: 153961 Human
see all -
别名
- Actin related protein 1 antibody
- Actin RPV antibody
- Actin-RPV antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARP1 antibody (ab11009)
Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat duodenum tissue labelling ARP1 with ab11009.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARP1 antibody (ab11009)Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat kidney tissue labelling ARP1 with ab11009.
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Western blot - Anti-ARP1 antibody (ab11009)All lanes : Anti-ARP1 antibody (ab11009) at 1/1000 dilution
Lane 1 : A432 cell lysate
Lane 2 : A432 cell lysate with ARP1 immunising peptide at 5 mg/ml
Predicted band size: 45 kDa
Observed band size: 45 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab11009 被引用在 2 文献中.
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580
- Kamal AHM et al. Cross-linking Proteomics Indicates Effects of Simvastatin on the TLR2 Interactome and Reveals ACTR1A as a Novel Regulator of the TLR2 Signal Cascade. Mol Cell Proteomics 18:1732-1744 (2019). PubMed: 31221720