Key features and details
- Goat polyclonal to ARIH1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
经测试应用适用于: WBmore details
HEGYEKDLWEYIED, corresponding to C terminal amino acids 544-557 of Human ARIH1.
- U937 lysate .
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
The Abpromise guarantee
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.
Optimal working dilutions / concentrations should be determined by the end user.
功能Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
序列相似性Belongs to the RBR family. Ariadne subfamily.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
细胞定位Cytoplasm. Mainly cytoplasmic.
- Information by UniProt
- Entrez Gene: 25820 Human
- Omim: 605624 Human
- SwissProt: Q9Y4X5 Human
- Unigene: 268787 Human
- ARI 1 antibody
- ARI antibody
- ARI-1 antibody
All lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution
Lanes 1-2 : NIH3T3 whole cell lysate at 30 µg
Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg
All lanes : Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution
Performed under reducing conditions.
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 15 minutes
Lanes 1 and 2 - 12% SDS-PAGE.
Lane 3 - 4-15% SDS-PAGE.
Lanes 1 and 3 - developed with colorimetric substrate.
Lane 2 - developed with ECF+.
ab3891 staining (0.5
µg/ml) of U937 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab3891 被引用在 5 文献中.
- Tan KL et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell 45:226-244.e8 (2018). PubMed: 29689197
- Polajnar M et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
- Scott DC et al. Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation. Cell 166:1198-1214.e24 (2016). PubMed: 27565346
- Wong ES et al. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem 282:12310-8 (2007). PubMed: 17329252
- LaVoie MJ et al. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem 103:2354-68 (2007). PubMed: 17883392