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Signal Transduction Metabolism Amino Acids

Anti-ARIH1抗体(ab3891)

  • Datasheet
Reviews (1) Submit a question References (5)

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Western blot - Anti-ARIH1 antibody (ab3891)
  • Western blot - Anti-ARIH1 antibody (ab3891)

Key features and details

  • Goat polyclonal to ARIH1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-ARIH1抗体
  • 描述

    山羊多克隆抗体to ARIH1
  • 宿主

    Goat
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide:

    HEGYEKDLWEYIED

    , corresponding to C terminal amino acids 544-557 of Human ARIH1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • U937 lysate .
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3891于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
说明
应用说明
Western Blot: Approx 65 kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735].
Recommended for use at 0.2-1.0µg/ml.
Not tested in other applications.

Optimal working dilutions / concentrations should be determined by the end user.

靶标

  • 功能

    Might act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates.
  • 组织特异性

    Widely expressed.
  • 序列相似性

    Belongs to the RBR family. Ariadne subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • 细胞定位

    Cytoplasm. Mainly cytoplasmic.
  • Target information above from: UniProt accession Q9Y4X5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 25820 Human
    • Omim: 605624 Human
    • SwissProt: Q9Y4X5 Human
    • Unigene: 268787 Human
    • 别名

      • ARI 1 antibody
      • ARI antibody
      • ARI-1 antibody
      • ARI1 antibody
      • ARI1_HUMAN antibody
      • Ariadne Drosophila homolog of antibody
      • Ariadne homolog antibody
      • Ariadne homolog ubiquitin conjugating enzyme E2 binding protein 1 antibody
      • Ariadne ubiquitin conjugating enzyme E2 binding protein homolog 1 antibody
      • ARIH 1 antibody
      • ARIH1 antibody
      • E3 ubiquitin protein ligase ARIH1 antibody
      • H7 AP2 antibody
      • H7-AP2 antibody
      • HARI antibody
      • HHARI antibody
      • human homologue of ariadne antibody
      • Monocyte protein 6 antibody
      • MOP 6 antibody
      • MOP-6 antibody
      • MOP6 antibody
      • Protein ariadne 1 homolog antibody
      • Protein ariadne-1 homolog antibody
      • Protein ariadne1 homolog antibody
      • UbcH7 binding protein antibody
      • UBCH7 BP antibody
      • UbcH7-binding protein antibody
      • UBCH7BP antibody
      • UbcM4 interacting protein antibody
      • UbcM4-interacting protein antibody
      • Ubiquitin conjugating enzyme E2 binding protein 1 antibody
      • Ubiquitin-conjugating enzyme E2-binding protein 1 antibody
      see all

    图片

    • Western blot - Anti-ARIH1 antibody (ab3891)
      Western blot - Anti-ARIH1 antibody (ab3891)This image is courtesy of an Abreview submitted by T Lawson
      All lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilution

      Lanes 1-2 : NIH3T3 whole cell lysate at 30 µg
      Lane 3 : Partially purified NIH3T3 protein mixture at 10 µg

      Secondary
      All lanes : Alkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilution

      Performed under reducing conditions.

      Observed band size: 65 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)


      Exposure time: 15 minutes


      Lanes 1 and 2 - 12% SDS-PAGE.

      Lane 3 - 4-15% SDS-PAGE.

      Lanes 1 and 3 - developed with colorimetric substrate.

      Lane 2 - developed with ECF+.

      See Abreview

    • Western blot - Anti-ARIH1 antibody (ab3891)
      Western blot - Anti-ARIH1 antibody (ab3891)
      ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab3891 staining (0.5µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab3891?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3891 被引用在 5 文献中.

    • Tan KL  et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell 45:226-244.e8 (2018). PubMed: 29689197
    • Polajnar M  et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
    • Scott DC  et al. Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation. Cell 166:1198-1214.e24 (2016). PubMed: 27565346
    • Wong ES  et al. Relative sensitivity of parkin and other cysteine-containing enzymes to stress-induced solubility alterations. J Biol Chem 282:12310-8 (2007). PubMed: 17329252
    • LaVoie MJ  et al. The effects of oxidative stress on parkin and other E3 ligases. J Neurochem 103:2354-68 (2007). PubMed: 17883392

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Western blot abreview for Anti-ARIH1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Mouse Cell lysate - whole cell (NIH3T3 cells)
    Specification
    NIH3T3 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. T Lawson

    Verified customer

    提交于 Sep 16 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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