Anti-ARID1B抗体[2D2] (ab57461)
![Western blot - Anti-ARID1B antibody [2D2] (ab57461)](https://www.abcam.cn/ps/products/57/ab57461/Images/ab57461-8-anti-arid1b-antibody-2d2-western-blot-human-hela-s3-ne.jpg "Western blot - Anti-ARID1B antibody [2D2] (ab57461)")
Key features and details
- Mouse monoclonal [2D2] to ARID1B
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-ARID1B抗体[2D2]
参阅全部 ARID1B 一抗 -
描述
小鼠单克隆抗体[2D2] to ARID1B -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: PPAKRHEGDM YNMQYSSQQQ EMYNQYGGSY SGPDRRPIQG QYPYPYSRER MQGPGQIQTH GIPPQMMGGP LQSSSSEGPQ QNMWAARNDM PYPYQNR, corresponding to amino acids 1364-1461 of Human ARID1B
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阳性对照
- ICC/IF: HepG2 cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 12 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
2D2 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab57461于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 236 kDa.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 236 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
ARID1B (AT-rich interactive domain-containing protein 1B) is a component of SWI/SNF chromatin remodeling complexes involved in transcriptional activation and repression of select genes by chromatin remodeling. It binds DNA non-specifically and alters DNA-nucleosome topology. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 57492 Human
- Omim: 614556 Human
- SwissProt: Q8NFD5 Human
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别名
- 6A3 5 antibody
- ARID 1B antibody
- ARID domain containing protein 1B antibody
see all
图片
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Western blot - Anti-ARID1B antibody [2D2] (ab57461)Anti-ARID1B antibody [2D2] (ab57461) at 5 µg/ml + HeLa (human cervix adenocarcinoma epithelial cell) Nuclear Extract at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 236 kDaBlocking and diluting buffer and concentration: 5% non-fat milk/PBST (0.2%).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1B antibody [2D2] (ab57461)](https://www.abcam.cn/ps/products/57/ab57461/Images/ab57461-6-anti-arid1b-antibody-2d2-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARID1B antibody [2D2] (ab57461)Immunoperoxidase analysis of formalin-fixed paraffin-embedded human kidney tissue labelling ARID1B with ab129165 at 3 µg/mL concentration.
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![Immunocytochemistry/ Immunofluorescence - Anti-ARID1B antibody [2D2] (ab57461)](https://www.abcam.cn/ps/products/57/ab57461/Images/ab57461-5-anti-arid1b-antibody-2d2-immunocytochemistry-immunofluorescence-human-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARID1B antibody [2D2] (ab57461)Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling ARID1B (Green) with ab129165 at 10 µg/mL concentration.
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![Sandwich ELISA - Anti-ARID1B antibody [2D2] (ab57461)](https://www.abcam.cn/ps/products/57/ab57461/Images/ab57461-7-anti-arid1b-antibody-2d2-sandwich-elisa-standard-curve.jpg)
Sandwich ELISA - Anti-ARID1B antibody [2D2] (ab57461)Sandwich ELSIA standard curve using ab57461 as a capture antibody. Detection limit for recombinant GST tagged ARID1B is 0.03 ng/mL.
实验方案
数据表及文件
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Datasheet download
文献 (28)
ab57461 被引用在 28 文献中.
- Vital T et al. MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing. Front Oncol 13:1099550 (2023). PubMed: 36793594
- Reddy D et al. Paraspeckles interact with SWI/SNF subunit ARID1B to regulate transcription and splicing. EMBO Rep 24:e55345 (2023). PubMed: 36354291
- Belk JA et al. Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence. Cancer Cell 40:768-786.e7 (2022). PubMed: 35750052
- Kim H et al. Early postnatal serotonin modulation prevents adult-stage deficits in Arid1b-deficient mice through synaptic transcriptional reprogramming. Nat Commun 13:5051 (2022). PubMed: 36030255
- Moffat JJ et al. Differential roles of ARID1B in excitatory and inhibitory neural progenitors in the developing cortex. Sci Rep 11:3856 (2021). PubMed: 33594090
