重组Anti-ARH抗体[EPR13115] - N-terminal (ab181043)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13115] to ARH - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ARH抗体[EPR13115] - N-terminal
参阅全部 ARH 一抗 -
描述
兔单克隆抗体[EPR13115] to ARH - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Humab fetal liver, K562 and 293T cell lysates. K562 cells; mouse heart and kidney and rat heart and kidney tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13115 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181043于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 34 kDa.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 34 kDa. |
靶标
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功能
Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. -
组织特异性
Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes. -
疾病相关
Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH) [MIM:603813]. ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) [MIM:143890] homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability. -
序列相似性
Contains 1 PID domain. -
结构域
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 26119 Human
- Entrez Gene: 100017 Mouse
- Entrez Gene: 500564 Rat
- Omim: 605747 Human
- SwissProt: Q5SW96 Human
- SwissProt: Q8C142 Mouse
- Unigene: 590911 Human
- Unigene: 482148 Mouse
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别名
- ARH antibody
- ARH GENE antibody
- ARH_HUMAN antibody
see all
图片
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All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysates
Lane 2 : Mouse kidney tissue lysates
Lane 3 : Rat heart tissue lysates
Lane 4 : Rat kidney tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1000 µg
Predicted band size: 34 kDa -
All lanes : Anti-ARH antibody [EPR13115] - N-terminal (ab181043) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysatae
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab181043 被引用在 1 文献中.
- Rodríguez-Jiménez C et al. A new variant (c.1A>G) in LDLRAP1 causing autosomal recessive hypercholesterolemia: Characterization of the defect and response to PCSK9 inhibition. Atherosclerosis N/A:N/A (2019). PubMed: 30777337