Anti-Argonaute-2抗体(ab5072)
Key features and details
- Rabbit polyclonal to Argonaute-2
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Argonaute-2抗体
参阅全部 Argonaute-2 一抗 -
描述
兔多克隆抗体to Argonaute-2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Drosophila melanogaster
不与反应: Human -
免疫原
Synthetic peptide corresponding to Drosophila melanogaster Argonaute-2 aa 550-650 conjugated to keyhole limpet haemocyanin.
(Peptide available asab24177) -
常规说明
Please note the immunogen for this antibody has no sequence alignment to human dIFC2. When tested in human, non-specific binding is observed.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 55, 95 kDa (predicted molecular weight: 106 kDa).
Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 55, 95 kDa (predicted molecular weight: 106 kDa). Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
靶标
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功能
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. -
序列相似性
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
结构域
The Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH). -
翻译后修饰
Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity. -
细胞定位
Cytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8. - Information by UniProt
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数据库链接
- Entrez Gene: 39683 Drosophila melanogaster
- SwissProt: Q9VUQ5 Drosophila melanogaster
- Unigene: 9677 Drosophila melanogaster
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别名
- Ago 2 antibody
- AGO2_HUMAN antibody
- Argonaute 2 antibody
see all
图片
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Western blot - Anti-Argonaute-2 antibody (ab5072)Anti-Argonaute-2 antibody (ab5072) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Additional bands at: 120 kDa, 19 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5072 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-Argonaute-2 antibody (ab5072)Anti-Argonaute-2 antibody (ab5072) at 1/500 dilution + Drosophila lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
Predicted band size: 106 kDa
Observed band size: 55,95 kDa why is the actual band size different from the predicted?Rabbit polyclonal to Ago2 (ab5072) at 1/500 on Drosophila lysate (20ug).
Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form.
Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.
Swissprot suggests Ago 2 may have 50kDa form.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab5072 被引用在 26 文献中.
- Xiong J et al. CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury. Cell Death Discov 8:315 (2022). PubMed: 35821018
- Liu L et al. CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. Mol Cancer 21:149 (2022). PubMed: 35858900
- Zhu Z et al. Hsa_circ_0016760 exacerbates the malignant development of non-small cell lung cancer by sponging miR-145-5p/FGF5. Oncol Rep 45:501-512 (2021). PubMed: 33416186
- Yan X et al. RAI14 Regulated by circNFATC3/miR-23b-3p axis Facilitates Cell Growth and Invasion in Gastric Cancer. Cell Transplant 30:9636897211007055 (2021). PubMed: 33840258
- Li D et al. Circular RNA SERPINE2 promotes development of glioblastoma by regulating the miR-361-3p/miR-324-5p/BCL2 signaling pathway. Mol Ther Oncolytics 22:483-494 (2021). PubMed: 34553034