Anti-Argininosuccinate Lyase抗体(ab97370)
Key features and details
- Rabbit polyclonal to Argininosuccinate Lyase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Argininosuccinate Lyase抗体
参阅全部 Argininosuccinate Lyase 一抗 -
描述
兔多克隆抗体to Argininosuccinate Lyase -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow
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免疫原
Recombinant fragment corresponding to Human Argininosuccinate Lyase aa 13-231.
Sequence:FVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQ ILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRS RNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQ RAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVD RELLRAELNFGAITLNSMDAPEATLGPCWGCSCGSDPGVGRAGLGGRPVA LLYRCCFCGEDHPRQGSILYSLLTSSKQTHVAPAAPEARPGGAWWDRSYF AQRPGGKEALPGGRATALLYRCCFCGEDHPQQGSTLYCVPTSTNQAQAAP EERPRAPWWDTSSGALRPVALKSPQVVCEAASAGLLKTLRFVKYLPCFQV LPLDQQLVLVRNCWASLLMLELAQDRLQFETVEVSEPSMLQKILTTRRRE TGGNEPLPVPTLQHHLAPPAEARKVPSASQVQAIKCFLSKCWSLNISTK
Database link: P04424 -
阳性对照
- WB: HepG2. HEK-293T, HeLa, A431 whole cell lysate. Neuro2a, GL261, C8D30, NIH/3T3, BCL-1, Raw 264.7, C2C12 whole cell lysate. ICC/IF: HeLa cells IHC-P: U-87 MG xenograft, C2C12 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97370于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| ICC/IF |
1/100 - 1/200.
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| IHC-P |
1/100 - 1/1000.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
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ICC/IF
1/100 - 1/200. |
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IHC-P
1/100 - 1/1000. |
靶标
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通路
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1. -
疾病相关
Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. -
序列相似性
Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. -
翻译后修饰
Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. - Information by UniProt
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数据库链接
- Entrez Gene: 435 Human
- Entrez Gene: 109900 Mouse
- Entrez Gene: 59085 Rat
- Omim: 608310 Human
- SwissProt: P04424 Human
- SwissProt: Q91YI0 Mouse
- SwissProt: P20673 Rat
- Unigene: 632015 Human
see all -
别名
- Argininosuccinase antibody
- Argininosuccinate lyase antibody
- Arginosuccinase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (ab97370)
Paraffin embedded C2C12 xenograft tissue stained for ASL using ab97370 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (ab97370)HeLa cells stained for ASL (green) using ab97370 at 1/500 dilution in ICC/IF.
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Western blot - Anti-Argininosuccinate Lyase antibody (ab97370)All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution
Lane 1 : Neuro2a whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lane 5 : BCL-1 whole cell lysate
Lane 6 : Raw 264.7 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (ab97370)Paraffin embedded U-87 MG xenograft tissue stained for ASL using ab97370 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Argininosuccinate Lyase antibody (ab97370)All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa10% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (ab97370)ab97370, at a 1/200 dilution, staining Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab97370 被引用在 7 文献中.
- Zhang W et al. The related mechanism of complete Freund's adjuvant-induced chronic inflammation pain based on metabolomics analysis. Biomed Chromatogr 35:e5020 (2021). PubMed: 33159321
- Lerner S et al. ASL expression in ALDH1A1+ neurons in the substantia nigra metabolically contributes to neurodegenerative phenotype. Hum Genet 140:1471-1485 (2021). PubMed: 34417872
- Drokhlyansky E et al. The Human and Mouse Enteric Nervous System at Single-Cell Resolution. Cell 182:1606-1622.e23 (2020). PubMed: 32888429
- Lerner S et al. ASL Metabolically Regulates Tyrosine Hydroxylase in the Nucleus Locus Coeruleus. Cell Rep 29:2144-2153.e7 (2019). PubMed: 31747589
- Lee JS et al. Urea Cycle Dysregulation Generates Clinically Relevant Genomic and Biochemical Signatures. Cell 174:1559-1570.e22 (2018). PubMed: 30100185
- Baruteau J et al. Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer. Nat Commun 9:3505 (2018). PubMed: 30158522
- Marini JC et al. The intestinal-renal axis for arginine synthesis is present and functional in the neonatal pig. Am J Physiol Endocrinol Metab 313:E233-E242 (2017). PubMed: 28611027