Anti-Argininosuccinate Lyase抗体(ab96215)
Key features and details
- Rabbit polyclonal to Argininosuccinate Lyase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Argininosuccinate Lyase抗体
参阅全部 Argininosuccinate Lyase 一抗 -
描述
兔多克隆抗体to Argininosuccinate Lyase -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow
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免疫原
Recombinant fragment corresponding to Human Argininosuccinate Lyase aa 86-335.
Database link: P04424 -
阳性对照
- WB: MOLT-4, HeLa, IHC-P: DLD1 xenograft. ICC/IF: HeLa.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96215于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
靶标
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通路
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1. -
疾病相关
Defects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. -
序列相似性
Belongs to the lyase 1 family. Argininosuccinate lyase subfamily. -
翻译后修饰
Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity. - Information by UniProt
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数据库链接
- Entrez Gene: 435 Human
- Entrez Gene: 109900 Mouse
- Omim: 608310 Human
- SwissProt: P04424 Human
- SwissProt: Q91YI0 Mouse
- Unigene: 632015 Human
- Unigene: 23869 Mouse
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别名
- Argininosuccinase antibody
- Argininosuccinate lyase antibody
- Arginosuccinase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argininosuccinate Lyase antibody (ab96215)
Paraffin embedded DLD1 xenograft stained for ASL using ab96215 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Argininosuccinate Lyase antibody (ab96215)HeLa cells stained for ASL (green) using ab96215 at 1/500 dilution in ICC/IF.
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Western blot - Anti-Argininosuccinate Lyase antibody (ab96215)All lanes : Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell extract
Lane 2 : Argininosuccinate Lyase Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa -
Western blot - Anti-Argininosuccinate Lyase antibody (ab96215)Anti-Argininosuccinate Lyase antibody (ab96215) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab96215 尚未被引用在任何文献中。