Anti-ARFGEF2/BIG2抗体(ab72420)
Key features and details
- Rabbit polyclonal to ARFGEF2/BIG2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ARFGEF2/BIG2抗体
参阅全部 ARFGEF2/BIG2 一抗 -
描述
兔多克隆抗体to ARFGEF2/BIG2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human ARFGEF2/BIG2. GenBank: NP_006411.1
Database link: Q9Y6D5 -
阳性对照
- HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72420 was affinity purified using an epitope specific to ARFGEF2/BIG2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72420于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 202 kDa (predicted molecular weight: 202 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 202 kDa (predicted molecular weight: 202 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. -
细胞定位
Cytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes. -
数据库链接
- Entrez Gene: 10564 Human
- Omim: 605371 Human
- SwissProt: Q9Y6D5 Human
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别名
- ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
- ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
- ARFGEF 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARFGEF2/BIG2 antibody (ab72420)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ARFGEF2/BIG with ab72420 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-ARFGEF2/BIG2 antibody (ab72420)All lanes : Anti-ARFGEF2/BIG2 antibody (ab72420) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 202 kDa
Observed band size: 202 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ARFGEF2/BIG2 antibody (ab72420)Detection of ARFGEF2/BIG by Western Blot of Immunprecipitate.
ab72420 at 1µg/ml staining ARFGEF2/BIG in HeLa whole cell lysate immunoprecipitated using ab72420 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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