重组Anti-ARF6抗体[EPR8357] (ab131261)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8357] to ARF6
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ARF6抗体[EPR8357]
参阅全部 ARF6 一抗 -
描述
兔单克隆抗体[EPR8357] to ARF6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: MCF7, 293T, HEK-293, HAP1, HeLa and A431 cell lysates; Rat kidney lysates. Flow Cyt: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8357 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200. Predicted molecular weight: 20 kDa.
For unpurified use at 1/1000 - 1/10000 dilution. |
说明 |
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WB
1/200. Predicted molecular weight: 20 kDa. For unpurified use at 1/1000 - 1/10000 dilution. |
靶标
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功能
GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension. -
序列相似性
Belongs to the small GTPase superfamily. Arf family. -
细胞定位
Golgi apparatus. Cell membrane. Endosome membrane. Cell membrane. Cell projection > filopodium membrane. Recruited to the cell membrane in association with CYTH2 and ARL4C. - Information by UniProt
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数据库链接
- Entrez Gene: 382 Human
- Entrez Gene: 11845 Mouse
- Entrez Gene: 79121 Rat
- Omim: 600464 Human
- SwissProt: P62330 Human
- SwissProt: P62331 Mouse
- SwissProt: P62332 Rat
- Unigene: 525330 Human
see all -
别名
- ADP ribosylation factor 6 antibody
- ADP ribosylation factor protein 6 antibody
- ADP-ribosylation factor 6 antibody
see all
图片
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Anti-ARF6 antibody [EPR8357] (ab131261) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This antibody shows low affinity on HeLa cells and detects a strong non-specific band around 75 KD.
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All lanes : Anti-ARF6 antibody [EPR8357] (ab131261) at 1/200 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse kidney lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Anti-ARF6 antibody [EPR8357] (ab131261) at 1/1000 dilution (Purified) + Rat kidney lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ARF6 knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131261 (unpurified) observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131261 was shown to recognize ARF6 when ARF6 knockout samples were used, along with additional cross-reactive bands. Wild-type and ARF6 knockout samples were subjected to SDS-PAGE. ab131261 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
All lanes : Anti-ARF6 antibody [EPR8357] (ab131261) at 1/1000 dilution ((unpurified))
Lane 1 : MCF7 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab131261 被引用在 8 文献中.
- Zhou G et al. Exosome Mediated Cytosolic Cisplatin Delivery Through Clathrin-Independent Endocytosis and Enhanced Anti-cancer Effect via Avoiding Endosome Trapping in Cisplatin-Resistant Ovarian Cancer. Front Med (Lausanne) 9:810761 (2022). PubMed: 35592860
- Dao TNT et al. Chimeric nanocomposites for the rapid and simple isolation of urinary extracellular vesicles. J Extracell Vesicles 11:e12195 (2022). PubMed: 35188341
- Zhang K et al. Long Non-Coding RNA MDFIC-7 Promotes Chordoma Progression Through Modulating the miR-525-5p/ARF6 Axis. Front Oncol 11:743718 (2021). PubMed: 34621682
- Liu J et al. Ethanol Extract of the Infructescence of Platycarya strobilacea Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells. Evid Based Complement Alternat Med 2020:2760979 (2020). PubMed: 32419796
- Guo L et al. Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion. Theranostics 10:6581-6598 (2020). PubMed: 32550891
- Costa Verdera H et al. Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis. J Control Release 266:100-108 (2017). WB . PubMed: 28919558
- Pelletán LE et al. ADP ribosylation factor 6 (ARF6) promotes acrosomal exocytosis by modulating lipid turnover and Rab3A activation. J Biol Chem 290:9823-41 (2015). IF . PubMed: 25713146
- Bailey TA et al. A kinase inhibitor screen reveals protein kinase C-dependent endocytic recycling of ErbB2 in breast cancer cells. J Biol Chem 289:30443-58 (2014). ICC/IF ; Human . PubMed: 25225290