AB32524

重组Anti-ARF1 + ARF3抗体[EP442Y]

Anti-ARF1 + ARF3 antibody [EP442Y]

BOND RX™ Validated
RabMAb
Recombinant
了解详情

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(7 Publications)

Rabbit Recombinant Monoclonal ARF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 7 publications.

查看别名

ADP-ribosylation factor 1, ARF1

8 Images

Flow Cytometry (Intracellular) - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Flow cytometry analysis of 4% paraformaldehyde fixed 90% methanol permeabilised MCF7 (human breast adenocarcinoma epithelial cell) cells using ab32524 at 1/500 dilution (red) and secondary Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution. The isotype control was Rabbit monoclonal IgG (ab172730) (Black) and the control was cell swithout incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Immunofluorescence staining of MCF7 cells with purified ab32524 working dilution of 1/500 counter-stained with DAPI. The secondary antibody was an Alexa Fluor^® 488 conjugated goat anti-rabbit (ab150077) used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control PBS was used instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32524 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Immunohistochemistry analysis of paraffin-embedded rat liver tissue sections using ab32524 at 1/2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on rat liver. The section was incubated with ab32524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Immunohistochemistry analysis of paraffin-embedded mouse liver tissue sections using ab32524 at 1/2000 dilution and ready to use secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on mouse liver. The section was incubated with ab32524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Lab

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Blocking and diluting buffer : 5% NFDM/TBST

Human ARF-1 recombinant protein, (Cat# : ab123538 )
Human ARF-3 recombinant protein, (Cat# : ab113581 )

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/20000 dilution

Lane 1:

Full length human ARF-1 recombinant protein at 0.015 µg

Lane 2:

Full length human ARF-3 recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 23 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Blocking and diluting buffer : 5% NFDM/TBST. Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/1000 dilution

All lanes:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Observed band size: 20 kDa

false

Exposure time: 70s

Lab

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (AB32524)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/1000 dilution

Lane 1:

SiHa (human cervical epithelial cell) whole cell lysate at 50 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa

false

Exposure time: 180s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP442Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen used for this product shares 100% homology with ARF3, ARF4 and ARF5. Cross-reactivity with the latter two protein have not been confirmed experimentally.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ARF1 and ARF3 also known as ADP-ribosylation factors 1 and 3 are small GTP-binding proteins belonging to the ARF family. ARF1 has a molecular mass of approximately 21 kDa. These proteins are expressed in most eukaryotic cells where they play roles in vesicular trafficking processes. ARF1 is mainly located in the Golgi apparatus while ARF3 appears in the plasma membrane and cytoplasm. They function as molecular switches by cycling between active GTP-bound and inactive GDP-bound states which regulates the formation and budding of vesicles.

Biological function summary

ARF1 and ARF3 are significant in the intracellular trafficking of proteins and lipids. The proteins are part of the coatomer protein complex (COPI) important for retrograde transport from the Golgi to the endoplasmic reticulum. By recruiting coat proteins to the Golgi membranes ARF1 and ARF3 facilitate the sorting and packaging of cargo molecules into transport vesicles. This process sustains the maintenance of organelle identity and the dynamics of membrane remodeling.

Pathways

ARF1 and ARF3 are involved in key cellular pathways particularly those related to vesicular trafficking and membrane dynamics. They participate in the COPI and clathrin-mediated pathways. Through these pathways ARF1 interacts with proteins like PDZD8 while ARF3 works with proteins involved in primary cilia formation. These interactions underline their integrative role in maintaining proper cellular organization and communication.

ARF1 and ARF3 have links to cancer and neurodegenerative diseases. Dysregulation of ARF1 is associated with various cancer types due to its role in cell proliferation and migration. ARF3 has connections to neurodegenerative disorders where altered vesicular trafficking can lead to pathological conditions. ARF1's interaction with the protein NFDM-442 highlights its importance in disease contexts whereby changes in these interactions might contribute to disease progression and severity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Small GTPase involved in protein trafficking between different compartments (PubMed : 8253837). Modulates vesicle budding and uncoating within the Golgi complex (PubMed : 8253837). In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane (PubMed : 8253837). The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles (PubMed : 8253837). The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD) (By similarity). Plays a key role in the regulation of intestinal stem cells and gut microbiota, and is essential for maintaining intestinal homeostasis (By similarity). Also plays a critical role in mast cell expansion but not in mast cell maturation by facilitating optimal mTORC1 activation (By similarity).. (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

See full target information ARF1

其他靶点

ADP-ribosylation factor 3

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 131: PubMed30054383

2018

Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vibha Singh,Chaitanya Erady,Nagaraj Balasubramanian

PloS one 9:e96647 PubMed24792215

2014

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.

Applications

Species

Mouse

Mark L Schultz,Luis Tecedor,Colleen S Stein,Mark A Stamnes,Beverly L Davidson

PloS one 9:e91675 PubMed24632837

2014

Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation.

Applications

Species

Human

Jeffrey D Covington,Jose E Galgani,Cedric Moro,Jamie M LaGrange,Zhengyu Zhang,Arild C Rustan,Eric Ravussin,Sudip Bajpeyi

The Journal of biological chemistry 287:3885-97 PubMed22158626

2011

AMF-26, a novel inhibitor of the Golgi system, targeting ADP-ribosylation factor 1 (Arf1) with potential for cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshimi Ohashi,Hiroshi Iijima,Noriyuki Yamaotsu,Kanami Yamazaki,Shigeo Sato,Mutsumi Okamura,Kenji Sugimoto,Shingo Dan,Shuichi Hirono,Takao Yamori

Journal of virology 85:2364-72 PubMed21177810

2010

Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds for antipoliovirus activity.

Applications

Species

Human

Minetaro Arita,Hirotatsu Kojima,Tetsuo Nagano,Takayoshi Okabe,Takaji Wakita,Hiroyuki Shimizu

BMC cell biology 10:58 PubMed19686593

2009

Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6.

Applications

Unspecified application

Species

Unspecified reactive species

Erin A Anthonio,Chantal Brees,Eveline Baumgart-Vogt,Tsunaki Hongu,Sofie J Huybrechts,Patrick Van Dijck,Guy P Mannaerts,Yasunori Kanaho,Paul P Van Veldhoven,Marc Fransen

The Journal of biological chemistry 282:30246-55 PubMed17693410

2007

p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of protease-activated receptor-2 by influence on receptor trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Weibo Luo,Yingfei Wang,Georg Reiser

View all publications

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