重组Anti-ARF1 + ARF3抗体[EPR443] (ab108347)
![Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)](https://www.abcam.cn/ps/products/108/ab108347/Images/ab108347-284168-anti-arf1-arf3-antibody-epr443-western-blot.jpg "Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR443] to ARF1 + ARF3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ARF1 + ARF3抗体[EPR443]
参阅全部 ARF1+ARF3 一抗 -
描述
兔单克隆抗体[EPR443] to ARF1 + ARF3 -
宿主
Rabbit -
特异性
The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally. -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal kidney, Human fetal brain and MCF7 lysates; Human breast ductal carcinoma, and Human colonic adenocarcinoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: CaCo2
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR443 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108347于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
靶标
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细胞定位
ARF1: Golgi apparatus. Cytoplasm > perinuclear region. ARF3: Golgi apparatus. Cytoplasm > perinuclear region. -
数据库链接
- Entrez Gene: 375 Human
- Entrez Gene: 377 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 11842 Mouse
- Entrez Gene: 140940 Rat
- Entrez Gene: 64310 Rat
- Omim: 103180 Human
- Omim: 103190 Human
see all
图片
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Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution
Lane 1 : Full length human ARF-1 recombinant protein
Lane 2 : Full length human ARF-3 recombinant protein
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM /TBST
Human ARF-1 recombinant protein, (Cat#:ab123538 )
Human ARF-3 recombinant protein, (Cat#:ab113581 ) -
![Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)](https://www.abcam.cn/ps/products/108/ab108347/Images/ab108347-210251-anti-arf1-arf3-antibody-epr443-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)](https://www.abcam.cn/ps/products/108/ab108347/Images/ab108347-120961-anti-arf1-arf3-antibody-epr443-western-blot.jpg)
Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)](https://www.abcam.cn/ps/products/108/ab108347/Images/ab108347-120960-anti-arf1-arf3-antibody-epr443-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)](https://www.abcam.cn/ps/products/108/ab108347/Images/ab108347-120959-anti-arf1-arf3-antibody-epr443-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)](https://www.abcam.cn/ps/products/108/ab108347/Images/ab108347-6-benefits-of-recombinant-antibodies.png)
Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108347 被引用在 7 文献中.
- Liu Q et al. Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2. J Pathol 253:106-118 (2021). PubMed: 33009820
- Liu J et al. ARF3 inhibits proliferation and promotes apoptosis in gastric cancer by regulating AKT and ERK pathway. Acta Biochim Pol 68:223-229 (2021). PubMed: 33847108
- Zhu T et al. TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis. Int Immunopharmacol 75:105836 (2019). PubMed: 31450153
- Rafiq NB et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol 216:181-197 (2017). WB . PubMed: 28007915
- Eiseler T et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem 291:462-77 (2016). PubMed: 26507660
- Schultz ML et al. CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events. PLoS One 9:e96647 (2014). WB ; Mouse . PubMed: 24792215
- Jung JJ et al. Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases. PLoS One 7:e44572 (2012). PubMed: 22962618