重组Anti-ARF GAP 2抗体[EPR8497] (ab133768)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8497] to ARF GAP 2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ARF GAP 2抗体[EPR8497]
参阅全部 ARF GAP 2 一抗 -
描述
兔单克隆抗体[EPR8497] to ARF GAP 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human ARF GAP 2 aa 300-400. The exact sequence is proprietary.
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阳性对照
- Human fetal liver, Human skeletal muscle, HepG2, Human fetal heart and HeLa whole cell lysate (ab150035); Human colon tissue and breast carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 7.80 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8497 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133768于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 56 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 56 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. -
序列相似性
Contains 1 Arf-GAP domain. -
细胞定位
Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment. - Information by UniProt
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数据库链接
- Entrez Gene: 84364 Human
- Entrez Gene: 77038 Mouse
- Entrez Gene: 362162 Rat
- Omim: 606908 Human
- SwissProt: Q8N6H7 Human
- SwissProt: Q99K28 Mouse
- SwissProt: Q3MID3 Rat
- Unigene: 436204 Human
see all -
别名
- ADP-ribosylation factor GTPase-activating protein 2 antibody
- ARF GAP 2 antibody
- ARFG2_HUMAN antibody
see all
图片
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All lanes : Anti-ARF GAP 2 antibody [EPR8497] (ab133768) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 56 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF GAP 2 antibody [EPR8497] (ab133768)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ARF GAP 2 with ab133768 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133768 被引用在 1 文献中.
- Yue X et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695