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AB155687

Anti-ARD1A 抗体

Anti-ARD1A antibody

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(2 Publications)

Rabbit Polyclonal ARD1A antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NAA10 aa 1 to C-terminus.

查看别名

ARD1, ARD1A, TE2, NAA10, N-alpha-acetyltransferase 10, N-terminal acetyltransferase complex ARD1 subunit homolog A, NatA catalytic subunit Naa10, hARD1

6 Images
Western blot - Anti-ARD1A antibody (AB155687)
  • WB

Unknown

Western blot - Anti-ARD1A antibody (AB155687)

12% SDS PAGE

All lanes:

Western blot - Anti-ARD1A antibody (ab155687) at 1/5000 dilution

Lane 1:

H1299 cell lysate, transfected with scramble siRNA at 30 µg

Lane 2:

H1299 cell lysate, transfected with ARDIA siRNA at 30 µg

Predicted band size: 26 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody (AB155687)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ARD1A antibody (AB155687)

Immunofluorescent analysis of methanol-fixed HeLa cells (100% for 5 mins) labeling ARD1A with ab155687 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARD1A antibody (AB155687)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARD1A antibody (AB155687)

Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue labeling ARD1A with ab155687 at 1/500 dilution.

Western blot - Anti-ARD1A antibody (AB155687)
  • WB

Supplier Data

Western blot - Anti-ARD1A antibody (AB155687)

Samples were separated by 12% SDS-PAGE

All lanes:

Western blot - Anti-ARD1A antibody (ab155687) at 1/1000 dilution

Lane 1:

293T cell lysates at 30 µg

Lane 2:

A431 cell lysates at 30 µg

Lane 3:

HeLa cell lysates at 30 µg

Lane 4:

HepG2 cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 26 kDa

false

Western blot - Anti-ARD1A antibody (AB155687)
  • WB

Supplier Data

Western blot - Anti-ARD1A antibody (AB155687)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-ARD1A antibody (ab155687) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

C8D30 whole cell lysates at 30 µg

Lane 3:

NIH 3T3 whole cell lysates at 30 µg

Lane 4:

Raw264.7 whole cell lysates at 30 µg

Lane 5:

C2C12 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 26 kDa

false

Immunoprecipitation - Anti-ARD1A antibody (AB155687)
  • IP

Supplier Data

Immunoprecipitation - Anti-ARD1A antibody (AB155687)

Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 µg of ab155687. Western blot analysis was performed using ab155687 diluted at 1/500.

All lanes:

Immunoprecipitation - Anti-ARD1A antibody (ab155687) at 5 µg

Lane 1:

HeLa whole cell extract

Lane 2:

HeLa whole cell extract with Control IgG

Lane 3:

HeLa whole cell extract with ab155687

Secondary

All lanes:

EasyBlot anti Rabbit IgG

Predicted band size: 26 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, IP, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human NAA10 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P41227

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ARD1A also known as NAA10 is a catalytic subunit of the N-terminal acetyltransferase complex. This protein has a mass of approximately 25 kDa. It acetylates the alpha-amino group of nascent polypeptides which affects stability localization and function of proteins. ARD1A is expressed in various tissues including heart skeletal muscle and liver. Its enzymatic activity requires a partnership with NAA15 another subunit which together form the N-terminal acetyltransferase A (NatA) complex.
Biological function summary

Acetylation by ARD1A modulates protein interactions and function. The NatA complex where ARD1A acts influences processes including cell cycle regulation and apoptosis. By modifying substrates critical for cell fate ARD1A plays a role in maintaining cellular balance. This complex helps in modifying proteins like actin which regulates the cytoskeleton a cellular structure essential for movement and division.

Pathways

Protein acetylation by ARD1A fits into the cellular signaling and protein degradation pathways. The complex alters substrates involved in proteasome-mediated degradation relating it to ubiquitin-proteasome pathways. Other interactions include the modulation of gene expression pathways impacting proteins like histones which are core to chromatin structure and gene regulation.

Improper ARD1A function can relate to cancer and neurodegenerative diseases. Alterations in ARD1A activity have connected it to tumorigenesis due to its role in cell growth regulation. Additionally decreased N-terminal acetylation by ARD1A has shown a link to neurodegeneration. This happens as it affects proteins such as tau which is significant in Alzheimer's disease when improperly modified.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed : 15496142, PubMed : 19420222, PubMed : 19826488, PubMed : 20145209, PubMed : 20154145, PubMed : 25489052, PubMed : 27708256, PubMed : 29754825, PubMed : 32042062). Acetylates amino termini that are devoid of initiator methionine (PubMed : 19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed : 12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed : 19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed : 20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed : 27708256). Acetylates HIST1H4A (PubMed : 29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed : 27422821).
See full target information NAA10
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34355692

2021

compensates for in mice in the amino-terminal acetylation pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hyae Yon Kweon,Mi-Ni Lee,Max Dorfel,Seungwoon Seo,Leah Gottlieb,Thomas PaPazyan,Nina McTiernan,Rasmus Ree,David Bolton,Andrew Garcia,Michael Flory,Jonathan Crain,Alison Sebold,Scott Lyons,Ahmed Ismail,Elaine Marchi,Seong-Keun Sonn,Se-Jin Jeong,Sejin Jeon,Shinyeong Ju,Simon J Conway,Taesoo Kim,Hyun-Seok Kim,Cheolju Lee,Tae-Young Roh,Thomas Arnesen,Ronen Marmorstein,Goo Taeg Oh,Gholson J Lyon

Oncotarget 7:2709-20 PubMed26646451

2015

microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10.

Applications

Unspecified application

Species

Unspecified reactive species

Hongju Yang,Qian Li,Jie Niu,Bai Li,Dejun Jiang,Zhihua Wan,Qingmei Yang,Fei Jiang,Ping Wei,Song Bai
View all publications
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