Anti-Aquaporin 2抗体(ab62628)
Key features and details
- Rabbit polyclonal to Aquaporin 2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Aquaporin 2抗体
参阅全部 Aquaporin 2 一抗 -
描述
兔多克隆抗体to Aquaporin 2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Aquaporin 2. Synthetic non-phosphopeptide derived from human Aquaporin 2 around the phosphorylation site of serine 256
Database link: P41181 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
组织特异性
Expressed in renal collecting tubules. -
疾病相关
Diabetes insipidus, nephrogenic, autosomal -
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
结构域
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
翻译后修饰
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. -
细胞定位
Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. - Information by UniProt
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数据库链接
- Entrez Gene: 359 Human
- Omim: 107777 Human
- SwissProt: P41181 Human
- Unigene: 130730 Human
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别名
- ADH water channel antibody
- AQP 2 antibody
- AQP CD antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 2 antibody (ab62628)Immunofluorescence analysis of HeLa cells, using 1/500-1/1000 ab62628.
Left image untreated.
Right image treated with peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab62628 被引用在 8 文献中.
- De Chiara L et al. Tubular cell polyploidy protects from lethal acute kidney injury but promotes consequent chronic kidney disease. Nat Commun 13:5805 (2022). PubMed: 36195583
- Zheng Y et al. Single-Cell Transcriptomics Reveal Immune Mechanisms of the Onset and Progression of IgA Nephropathy. Cell Rep 33:108525 (2020). PubMed: 33357427
- Shen X et al. Transparenchymal Renal Pelvis Injection of Recombinant Adeno-Associated Virus Serotype 9 Vectors Is a Practical Approach for Gene Delivery in the Kidney. Hum Gene Ther Methods 29:251-258 (2018). PubMed: 30458119
- Wan S et al. Estrogen nuclear receptors affect cell migration by altering sublocalization of AQP2 in glioma cell lines. Cell Death Discov 4:49 (2018). PubMed: 30345080
- Baturina GS et al. Brattleboro rats have impaired apical membrane water permeability regulation in the outer medullary collecting duct principal cells. Clin Exp Pharmacol Physiol 43:1225-1233 (2016). PubMed: 27593225
- Landegren N et al. Autoantibodies Targeting a Collecting Duct-Specific Water Channel in Tubulointerstitial Nephritis. J Am Soc Nephrol 27:3220-3228 (2016). PubMed: 26984885
- Kang HM et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). PubMed: 26776520
- Steele SL et al. Telomerase immortalization of principal cells from mouse collecting duct. Am J Physiol Renal Physiol 299:F1507-14 (2010). IF ; Mouse . PubMed: 20926633