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Signal Transduction Metabolism Plasma Membrane Channels

Anti-Aquaporin 1抗体(ab15080)

  • Datasheet
Reviews (17)Q&A (7)References (68)

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ELISA - Anti-Aquaporin 1 antibody (ab15080)

    Key features and details

    • Rabbit polyclonal to Aquaporin 1
    • Suitable for: ELISA
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Aquaporin 1抗体
      参阅全部 Aquaporin 1 一抗
    • 描述

      兔多克隆抗体to Aquaporin 1
    • 宿主

      Rabbit
    • 经测试应用

      适用于: ELISAmore details
    • 免疫原

      A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituent: 100% Whole serum
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Metabolism
      • Plasma Membrane
      • Channels
      • Metabolism
      • Types of disease
      • Cancer
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Aquaporin 1 protein (ab114210)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab15080于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ELISA
    Use at an assay dependent concentration.
    说明
    ELISA
    Use at an assay dependent concentration.

    靶标

    • 功能

      Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
    • 组织特异性

      Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
    • 序列相似性

      Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • 结构域

      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession P29972 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 别名

      • AQP 1 antibody
      • AQP CHIP antibody
      • AQP-1 antibody
      • AQP1 antibody
      • AQP1_HUMAN antibody
      • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
      • aquaporin 1 (Colton blood group) antibody
      • Aquaporin CHIP antibody
      • Aquaporin-1 antibody
      • Aquaporin-CHIP antibody
      • Aquaporin1 antibody
      • Channel forming integral protein 28kDa antibody
      • Channel like integral membrane protein 28 kDa antibody
      • CHIP 28 antibody
      • CHIP28 antibody
      • CO antibody
      • Colton blood group antibody
      • Growth factor induced delayed early response protein antibody
      • MGC26324 antibody
      • Urine water channel antibody
      • Water channel protein CHIP 29 antibody
      • Water channel protein CHIP29 antibody
      • Water channel protein for red blood cells and kidney proximal tubule antibody
      see all

    图片

    • ELISA - Anti-Aquaporin 1 antibody (ab15080)
      ELISA - Anti-Aquaporin 1 antibody (ab15080)

      Antibody titer determination against Aquaporin-1 using pre-immunised serum, Antiserum and Affinity purified Anti-Aquaporin 1 (Cat: ab15080).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (68)

    发表研究结果有使用 ab15080?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab15080 被引用在 68 文献中.

    • Parascandolo A  et al. Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins. Stem Cells Int 2023:8344259 (2023). PubMed: 37223543
    • Kimball EC  et al. Aquaporin 4 is not present in normal porcine and human lamina cribrosa. PLoS One 17:e0268541 (2022). PubMed: 35709078
    • Rappold PM  et al. A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear-Cell Renal Cell Carcinoma. Cancer Discov 12:2308-2329 (2022). PubMed: 35758895
    • Tofteng SS  et al. Increased COX-2 after ureter obstruction attenuates fibrosis and is associated with EP2 receptor upregulation in mouse and human kidney. Acta Physiol (Oxf) 235:e13828 (2022). PubMed: 35543087
    • Fan Z  et al. Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4+ endothelial cells. Cell Metab 33:1793-1807.e9 (2021). PubMed: 34358431
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 24 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Aquaporin 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Choroid Plexus organoid, Day 67)
    Permeabilization
    Yes - 0.3% TritonX
    Specification
    Choroid Plexus organoid, Day 67
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 16 2024

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0 (ab64236 )
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 23 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 28 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Kidney
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 28 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Aquaporin 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Kidney primary culture)
    Permeabilization
    No
    Specification
    Kidney primary culture
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 07 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Liver
    Blocking step
    sea blcok as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. David Ivancic

    Verified customer

    提交于 Jan 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    kidney
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Aquaporin 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Colon
    Blocking step
    sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. David Ivancic

    Verified customer

    提交于 Jul 02 2019

    1-10 of 24 Abreviews or Q&A

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