Anti-Aquaporin 1抗体[1/22] (ab9566)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.cn/ps/products/9/ab9566/Images/ab9566-17269-anti-aquaporin-1-antibody-1-22-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)")
Key features and details
- Mouse monoclonal [1/22] to Aquaporin 1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
概述
-
产品名称
Anti-Aquaporin 1抗体[1/22]
参阅全部 Aquaporin 1 一抗 -
描述
小鼠单克隆抗体[1/22] to Aquaporin 1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Rat, Human
预测可用于: Sheep, Cow
-
免疫原
Synthetic peptide:
GQVEEYDLDDDINSRVEMKPK
, corresponding to amino acids 249-269 of Aquaporin 1. -
表位
Intracellular C-terminal AQP 1 epitope (active channel loop) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
1/22 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
|
| IHC-P | (11) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1-2µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. -
组织特异性
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver. -
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
结构域
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 358 Human
- Entrez Gene: 25240 Rat
- Omim: 107776 Human
- SwissProt: P29972 Human
- SwissProt: P29975 Rat
- Unigene: 76152 Human
- Unigene: 1618 Rat
-
别名
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical staining of aquaporin 1 in rat brain.
-
![Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.cn/ps/products/9/ab9566/Images/ab9566-17267-anti-aquaporin-1-antibody-1-22-flow-cytometry.jpg)
Flow Cytometry - Anti-Aquaporin 1 antibody [1/22] (ab9566)Overlay histogram showing HEK293 cells stained with ab9566 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9566, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.cn/ps/products/9/ab9566/Images/ab9566-279831-anti-aquaporin-1-antibody-1-22-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 1 antibody [1/22] (ab9566)Immunohistochemical analysis of exocrine pancreas cells labeling Aquaporin 1 with ab9566.
-
![Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)](https://www.abcam.cn/ps/products/9/ab9566/Images/ab9566-17268-anti-aquaporin-1-antibody-1-22-western-blot.jpg)
Western blot - Anti-Aquaporin 1 antibody [1/22] (ab9566)Anti-Aquaporin 1 antibody [1/22] (ab9566) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 50 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (68)
ab9566 被引用在 68 文献中.
- Chen PP et al. Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease. Theranostics 13:3988-4003 (2023). PubMed: 37554279
- Onishi Y et al. Development of Urinary Diagnostic Biomarker for IgA Nephropathy by Lectin Microarray. Am J Nephrol 53:10-20 (2022). PubMed: 34965524
- Krohn P et al. Multisystem involvement, defective lysosomes and impaired autophagy in a novel rat model of nephropathic cystinosis. Hum Mol Genet 31:2262-2278 (2022). PubMed: 35137071
- Li Q et al. Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development. Cell Death Differ 29:1596-1610 (2022). PubMed: 35322202
- Silva-Aguiar RP et al. SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells. Biochim Biophys Acta Mol Basis Dis 1868:166496 (2022). PubMed: 35863591