Anti-AQP0抗体(ab134695)
Key features and details
- Rabbit polyclonal to AQP0
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AQP0抗体 -
描述
兔多克隆抗体to AQP0 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human AQP0 (internal sequence).
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阳性对照
- HT29 cell extract; Human brain tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 28 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 28 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Water channel. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core. -
组织特异性
Major component of lens fiber gap junctions. -
疾病相关
Cataract 15, multiple types -
序列相似性
Belongs to the MIP/aquaporin (TC 1.A.8) family. -
结构域
Aquaporins contain two tandem repeats each containing two membrane-spanning helices and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). Each tandem repeat contains a loop and a short helix that enter and leave the lipid bilayer on the same side. -
翻译后修饰
Subject to partial proteolytic cleavage in the eye lens core. Partial proteolysis promotes interactions between tetramers from adjoining membranes. -
细胞定位
Cell membrane. Cell junction > gap junction. - Information by UniProt
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数据库链接
- Entrez Gene: 4284 Human
- Entrez Gene: 17339 Mouse
- Entrez Gene: 25480 Rat
- Omim: 154050 Human
- SwissProt: P30301 Human
- SwissProt: P51180 Mouse
- SwissProt: P09011 Rat
- Unigene: 574026 Human
see all -
别名
- AQP 0 antibody
- AQP0 antibody
- Aquaporin antibody
see all
图片
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Western blot - Anti-AQP0 antibody (ab134695)Anti-AQP0 antibody (ab134695) at 1/500 dilution + HT29 cell extract at 30 µg
Predicted band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AQP0 antibody (ab134695)Immunohistochemical analysis of AQP0 in paraffin-embedded Human brain tissue using ab134695 at 1/50 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab134695 被引用在 3 文献中.
- Binch A et al. Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues. JOR Spine 3:e1098 (2020). PubMed: 33015573
- Bradley JR et al. Signaling through tumor necrosis receptor 2 induces stem cell marker in CD133+ regenerating tubular epithelial cells in acute cell-mediated rejection of human renal allografts. Am J Transplant 20:2380-2391 (2020). PubMed: 32167668
- Snuggs JW et al. Aquaporin expression in the human and canine intervertebral disc during maturation and degeneration. JOR Spine 2:e1049 (2019). PubMed: 31463463