重组Anti-Apolipoprotein L1/APOL1抗体[EPR2907(2)] (ab108315)
![Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.cn/ps/products/108/ab108315/Images/ab108315-120870-anti-apolipoprotein-l-1-antibody-epr29072-western-blot.jpg "Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2907(2)] to Apolipoprotein L1/APOL1
- Suitable for: WB, IP, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Apolipoprotein L1/APOL1抗体[EPR2907(2)]
参阅全部 Apolipoprotein L1/APOL1 一抗 -
描述
兔单克隆抗体[EPR2907(2)] to Apolipoprotein L1/APOL1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, Flow Cyt (Intra), ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human plasma, ovary cancer, and HepG2 cell lysates. ICC/IF: BxPC-3 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2907(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108315于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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| IP |
1/10 - 1/100.
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/50.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
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IP
1/10 - 1/100. |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
1/50. |
靶标
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功能
May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. -
组织特异性
Plasma. Found on APOA-I-containing high density lipoprotein (HDL3). Expressed in pancreas, lung, prostate, liver, placenta and spleen. -
疾病相关
Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4) [MIM:612551]. It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
序列相似性
Belongs to the apolipoprotein L family. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 8542 Human
- Omim: 603743 Human
- SwissProt: O14791 Human
- Unigene: 114309 Human
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别名
- APO L antibody
- Apo-L antibody
- ApoL antibody
see all
图片
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Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)All lanes : Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.cn/ps/products/108/ab108315/Images/ab108315-4-anti-apolipoprotein-l1-apol1-antibody-epr29072-immunocytochemistry-bxpc3-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labeling Apolipoprotein L1/APOL1 with purified ab108315 at 1/50 dilution (9.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.cn/ps/products/108/ab108315/Images/ab108315-257551-anti-apolipoprotein-l-1-antibody-epr29072-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.cn/ps/products/108/ab108315/Images/ab108315-257552-anti-apolipoprotein-l-1-antibody-epr29072-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.
Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.
Lane 1:Human plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)](https://www.abcam.cn/ps/products/108/ab108315/Images/ab108315-5-benefits-of-recombinant-antibodies.png)
Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab108315 被引用在 15 文献中.
- Zhao X et al. Chrysophanol Ameliorates Hemin-Induced Oxidative Stress and Endoplasmic Reticulum Stress by Regulating MicroRNA-320-5p/Wnt3a Pathway in HT22 Cells. Oxid Med Cell Longev 2022:9399658 (2022). PubMed: 35936221
- Lu HY et al. Knockdown of lncRNA MALAT1 ameliorates acute kidney injury by mediating the miR-204/APOL1 pathway. J Clin Lab Anal 35:e23881 (2021). PubMed: 34240756
- Divers J et al. Genome-wide association study for time to failure of kidney transplants from African American deceased donors. Clin Transplant 34:e13827 (2020). PubMed: 32080893
- O'Toole JF et al. ApoL1 Overexpression Drives Variant-Independent Cytotoxicity. J Am Soc Nephrol 29:869-879 (2018). PubMed: 29180397
- Li YL et al. Screening and identification of differentially expressed serum proteins in patients with vitiligo using two-dimensional gel electrophoresis coupled with mass spectrometry. Mol Med Rep 17:2651-2659 (2018). PubMed: 29207142