AB108315

重组Anti-Apolipoprotein L1/APOL1抗体[EPR2907(2)]

Name: Apolipoprotein L1/APOL1抗体[EPR2907(2)] (ab108315)| Abcam中文官网
Brand: Abcam Limited
SKU: AB108315
Price: 2938 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)]

RabMAb
Recombinant
了解详情

(1 Review)

(17 Publications)

Rabbit Recombinant Monoclonal Apolipoprotein L1/APOL1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 17 publications.

查看别名

APOL, APOL1, Apolipoprotein L1, Apolipoprotein L, Apolipoprotein L-I, Apo-L, ApoL, ApoL-I

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315)

Immunocytochemistry/ Immunofluorescence analysis of BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labeling Apolipoprotein L1/APOL1 with purified ab108315 at 1/50 dilution (9.2 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human hepatocellular carcinoma) cell line labeling Apolipoprotein L 1/APOL1 with ab108315 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Green). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315)

Apolipoprotein L 1/APOL1 was immunoprecipitated from 1mg of Human plasma with ab108315 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab108315 at 1/1000 dilution.

Goat Anti-Rabbit IgG peroxidase conjugated,was used as secondary antibody at 1/1000 dilution.

Lane 1 : Human plasma

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315)

Predicted band size: 44 kDa

Observed band size: 39 kDa,42 kDa

false

Unknown

Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (AB108315)

All lanes:

Western blot - Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] (ab108315) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human ovary cancer lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Predicted band size: 44 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2907(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein L1 also known as APOL1 is a protein with a mass of approximately 42 kDa. It is primarily expressed in the liver but can also be found in various tissues including the placenta pancreas and kidney. As a part of the apolipoprotein family APOL1 contributes to lipid binding and transport processes. The protein is involved in innate immunity due to its ability to bind and kill certain trypanosomes which are parasites that can cause disease in humans.

Biological function summary

APOL1 functions as a component in lipid transport and immune response. It plays an important role in the lytic pathway against trypanosomes by forming a pore in their lysosomal membranes leading to cell death. While it does not form part of a larger complex its activity is important when interacting with other factors and lipids. APOL1's actions demonstrate an integral aspect of the body's defense system particularly with its influence on cellular structures through lipid interactions.

Pathways

APOL1 is involved in the regulation of lipid metabolism and immune response pathways. It plays a role in the high-density lipoprotein (HDL) particle remodeling pathway. Another significant pathway involving APOL1 is the APOL family protein pathway which includes related proteins like APOL2 and APOL3. These proteins collaboratively contribute to intracellular lipid transport and immune defense establishing an interconnected network among them.

There is a significant connection between APOL1 variants and chronic kidney disease (CKD) and non-diabetic forms of End-Stage Renal Disease (ESRD). The risk alleles of the APOL1 gene are associated with increased incidence of CKD especially in individuals of African descent. These associations highlight the interaction between APOL1 and renal pathology as well as its linkage to other proteins like MYH9 which also relate to kidney diseases. Understanding APOL1's role in these conditions may aid in developing targeted medical interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. A component of trypanosome lytic factor of human serum; plays a crucial role in killing Trypanosoma brucei by forming pores in parasite lysosomal membranes and sensitizing T.brucei to oxidation-stimulated osmotic lysis (PubMed : 12621437, PubMed : 16020735, PubMed : 19997494, PubMed : 26645690, PubMed : 7723792).

See full target information APOL1

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 47:2553384 PubMed40948471

2025

A novel membrane-addressing domain mutation () in Chinese twins with FSGS: implications for podocyte injury and ion channel disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Peng,Dan Chang,Tingyu Chen,Sipei Chen,Li Wang,Guisen Li

Journal of experimental & clinical cancer research : CR 43:29 PubMed38263248

2024

HIF-2α/LINC02609/APOL1-mediated lipid storage promotes endoplasmic reticulum homeostasis and regulates tumor progression in clear-cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haibing Xiao,Yan Qu,Haolin Li,Yi Zhang,Mintian Fei,Chaozhao Liang,Hongmei Yang,Xiaoping Zhang

Oxidative medicine and cellular longevity 2022:9399658 PubMed35936221

2022

Chrysophanol Ameliorates Hemin-Induced Oxidative Stress and Endoplasmic Reticulum Stress by Regulating MicroRNA-320-5p/Wnt3a Pathway in HT22 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhao,Dongge Qiao,Dongsheng Guan,Kun Wang,Yinglin Cui

Journal of clinical laboratory analysis 35:e23881 PubMed34240756

2021

Knockdown of lncRNA MALAT1 ameliorates acute kidney injury by mediating the miR-204/APOL1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Yuan Lu,Guo-Yi Wang,Jin-Wen Zhao,Hai-Tao Jiang

Clinical transplantation 34:e13827 PubMed32080893

2020

Genome-wide association study for time to failure of kidney transplants from African American deceased donors.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Divers,Lijun Ma,William Mark Brown,Nicholette D Palmer,Young Choi,Ajay K Israni,Stephen O Pastan,Bruce A Julian,Robert S Gaston,Pamela J Hicks,Amber M Reeves-Daniel,Barry I Freedman

Molecular medicine reports 17:2651-2659 PubMed29207142

2017

Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Lei Li,Rui-Qun Qi,Yang Yang,He-Xiao Wang,Hang-Hang Jiang,Zheng-Xiu Li,Bi-Huan Xiao,Song Zheng,Yu-Xiao Hong,Jiu-Hong Li,Hong-Duo Chen,Xing-Hua Gao

Journal of the American Society of Nephrology : JASN 29:869-879 PubMed29180397

2017

ApoL1 Overexpression Drives Variant-Independent Cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

John F O'Toole,William Schilling,Diana Kunze,Sethu M Madhavan,Martha Konieczkowski,Yaping Gu,Liping Luo,Zhenzhen Wu,Leslie A Bruggeman,John R Sedor

Nature medicine 23:945-953 PubMed28650456

2017

A tripartite complex of suPAR, APOL1 risk variants and αβ integrin on podocytes mediates chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Salim S Hayek,Kwi Hye Koh,Morgan E Grams,Changli Wei,Yi-An Ko,Jing Li,Beata Samelko,Hyun Lee,Ranadheer R Dande,Ha Won Lee,Eunsil Hahm,Vasil Peev,Melissa Tracy,Nicholas J Tardi,Vineet Gupta,Mehmet M Altintas,Garrett Garborcauskas,Nikolina Stojanovic,Cheryl A Winkler,Michael S Lipkowitz,Adrienne Tin,Lesley A Inker,Andrew S Levey,Martin Zeier,Barry I Freedman,Jeffrey B Kopp,Karl Skorecki,Josef Coresh,Arshed A Quyyumi,Sanja Sever,Jochen Reiser

Journal of the American Society of Nephrology : JA 28:1093-1105 PubMed27821631

2016

Renal-Risk Variants Induce Mitochondrial Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Ma,Jeff W Chou,James A Snipes,Manish S Bharadwaj,Ann L Craddock,Dongmei Cheng,Allison Weckerle,Snezana Petrovic,Pamela J Hicks,Ashok K Hemal,Gregory A Hawkins,Lance D Miller,Anthony J A Molina,Carl D Langefeld,Mariana Murea,John S Parks,Barry I Freedman

Journal of lipid research 57:1059-73 PubMed27112635

2016

ApoL1 levels in high density lipoprotein and cardiovascular event presentation in patients with familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Judit Cubedo,Teresa Padró,Rodrigo Alonso,Pedro Mata,Lina Badimon

View all publications

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重组Anti-Apolipoprotein L1/APOL1抗体[EPR2907(2)]